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Segment-specific optogenetic stimulation in Drosophila melanogaster with linear arrays of organic light-emitting diodes
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dc.contributor.author | Murawski, Caroline | |
dc.contributor.author | Pulver, Stefan Robert | |
dc.contributor.author | Gather, Malte C. | |
dc.date.accessioned | 2020-12-07T15:58:07Z | |
dc.date.available | 2020-12-07T15:58:07Z | |
dc.date.issued | 2020-12-07 | |
dc.identifier | 270944615 | |
dc.identifier | bc18af4b-8d2d-4d94-91af-3b52ba2a36d1 | |
dc.identifier | 85097267145 | |
dc.identifier | 000598906000011 | |
dc.identifier.citation | Murawski , C , Pulver , S R & Gather , M C 2020 , ' Segment-specific optogenetic stimulation in Drosophila melanogaster with linear arrays of organic light-emitting diodes ' , Nature Communications , vol. 11 , 6248 . https://doi.org/10.1038/s41467-020-20013-6 | en |
dc.identifier.issn | 2041-1723 | |
dc.identifier.other | ORCID: /0000-0002-4857-5562/work/85168044 | |
dc.identifier.other | ORCID: /0000-0001-5170-7522/work/85168318 | |
dc.identifier.uri | https://hdl.handle.net/10023/21092 | |
dc.description | This research was financially supported by the EPSRC NSF-CBET lead agency agreement (EP/R010595/1, 1706207), the DARPA-NESD programme (N66001-17-C-4012) and the Leverhulme Trust (RPG-2017-231). C.M. acknowledges funding from the European Commission through a Marie Skłodowska Curie individual fellowship (703387). S.R.P acknowledges funding from the Biology and Biotechnology Research council (BB/M021793). M.C.G. acknowledges funding from the Alexander von Humboldt Stiftung (Humboldt-Professorship). | en |
dc.description.abstract | Optogenetics allows light-driven, non-contact control of neural systems, but light delivery remains challenging, in particular when fine spatial control of light is required to achieve local specificity. Here, we employ organic light-emitting diodes (OLEDs) that are micropatterned into linear arrays to obtain precise optogenetic control in Drosophila melanogaster larvae expressing the light-gated activator CsChrimson and the inhibitor GtACR2 within their peripheral sensory system. Our method allows confinement of light stimuli to within individual abdominal segments, which facilitates the study of larval behaviour in response to local sensory input. We show controlled triggering of specific crawling modes and find that targeted neurostimulation in abdominal segments switches the direction of crawling. More broadly, our work demonstrates how OLEDs can provide tailored patterns of light for photo-stimulation of neuronal networks, with future implications ranging from mapping neuronal connectivity in cultures to targeted photo-stimulation with pixelated OLED implants in vivo. | |
dc.format.extent | 11 | |
dc.format.extent | 2386611 | |
dc.language.iso | eng | |
dc.relation.ispartof | Nature Communications | en |
dc.subject | QC Physics | en |
dc.subject | QH301 Biology | en |
dc.subject | DAS | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | QH301 | en |
dc.title | Segment-specific optogenetic stimulation in Drosophila melanogaster with linear arrays of organic light-emitting diodes | en |
dc.type | Journal article | en |
dc.contributor.sponsor | European Commission | en |
dc.contributor.sponsor | US Department of Defence | en |
dc.contributor.sponsor | BBSRC | en |
dc.contributor.institution | University of St Andrews. Centre for Biophotonics | en |
dc.contributor.institution | University of St Andrews. School of Psychology and Neuroscience | en |
dc.contributor.institution | University of St Andrews. Sir James Mackenzie Institute for Early Diagnosis | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.identifier.doi | 10.1038/s41467-020-20013-6 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2020-12-06 | |
dc.identifier.url | https://doi.org/10.1101/2020.03.24.005090 | en |
dc.identifier.grantnumber | 703387 | en |
dc.identifier.grantnumber | N66001-17-C-4012 | en |
dc.identifier.grantnumber | BB/M021793/1 | en |
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