Segment-specific optogenetic stimulation in Drosophila melanogaster with linear arrays of organic light-emitting diodes
MetadataShow full item record
Optogenetics allows light-driven, non-contact control of neural systems, but light delivery remains challenging, in particular when fine spatial control of light is required to achieve local specificity. Here, we employ organic light-emitting diodes (OLEDs) that are micropatterned into linear arrays to obtain precise optogenetic control in Drosophila melanogaster larvae expressing the light-gated activator CsChrimson and the inhibitor GtACR2 within their peripheral sensory system. Our method allows confinement of light stimuli to within individual abdominal segments, which facilitates the study of larval behaviour in response to local sensory input. We show controlled triggering of specific crawling modes and find that targeted neurostimulation in abdominal segments switches the direction of crawling. More broadly, our work demonstrates how OLEDs can provide tailored patterns of light for photo-stimulation of neuronal networks, with future implications ranging from mapping neuronal connectivity in cultures to targeted photo-stimulation with pixelated OLED implants in vivo.
Murawski , C , Pulver , S R & Gather , M C 2020 , ' Segment-specific optogenetic stimulation in Drosophila melanogaster with linear arrays of organic light-emitting diodes ' , Nature Communications , vol. 11 , 6248 . https://doi.org/10.1038/s41467-020-20013-6
Copyright © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
DescriptionThis research was financially supported by the EPSRC NSF-CBET lead agency agreement (EP/R010595/1, 1706207), the DARPA-NESD programme (N66001-17-C-4012) and the Leverhulme Trust (RPG-2017-231). C.M. acknowledges funding from the European Commission through a Marie Skłodowska Curie individual fellowship (703387). S.R.P acknowledges funding from the Biology and Biotechnology Research council (BB/M021793). M.C.G. acknowledges funding from the Alexander von Humboldt Stiftung (Humboldt-Professorship).
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.