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Lipidomic profiling of plasma free fatty acids in type-1 diabetes highlights specific changes in lipid metabolism
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dc.contributor.author | Sobczak, Amelie Isabelle Sylvie | |
dc.contributor.author | Pitt, Samantha J. | |
dc.contributor.author | Smith, Terry K | |
dc.contributor.author | Ajjan, Ramzi A. | |
dc.contributor.author | Stewart, Alan J. | |
dc.date.accessioned | 2020-10-09T15:30:16Z | |
dc.date.available | 2020-10-09T15:30:16Z | |
dc.date.issued | 2021-01 | |
dc.identifier | 270305140 | |
dc.identifier | 6c163be1-71ee-48ff-8e2d-8fbacc478344 | |
dc.identifier | 85092304864 | |
dc.identifier | 000592664200012 | |
dc.identifier.citation | Sobczak , A I S , Pitt , S J , Smith , T K , Ajjan , R A & Stewart , A J 2021 , ' Lipidomic profiling of plasma free fatty acids in type-1 diabetes highlights specific changes in lipid metabolism ' , Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids , vol. 1866 , no. 1 , 158823 . https://doi.org/10.1016/j.bbalip.2020.158823 | en |
dc.identifier.issn | 1388-1981 | |
dc.identifier.other | ORCID: /0000-0003-4580-1840/work/81797587 | |
dc.identifier.other | ORCID: /0000-0003-2257-1595/work/81797623 | |
dc.identifier.uri | https://hdl.handle.net/10023/20755 | |
dc.description | This research was funded by the British Heart Foundation [grant numbers PG/15/9/31270, FS/15/42/3155]. | en |
dc.description.abstract | Type-1 diabetes mellitus (T1DM) is associated with metabolic changes leading to alterations in glucose and lipid handling. While T1DM- associated effects on many major plasma lipids have been characterised, such effects on plasma free fatty acids (FFA) have not been fully examined. Using gas chromatography-mass spectrometry, we measured the plasma concentrations of FFA species in individuals with T1DM (n=44) and age/sex-matched healthy controls (n=44). Relationships between FFA species and various parameters were evaluated. Plasma concentrations of myristate (14:0), palmitoleate (16:1), palmitate (16:0), linoleate (18:2), oleate (18:1c9), cis-vaccenate (18:1c11), eicosapentaenoate (20:5), arachidonate (20:4) and docosahexanoate (22:6) were reduced in the T1DM group (p<0.0001 for all, except p=0.0020 for eicosapentaenoate and p=0.0068 for arachidonate); α-linolenate (18:3) and dihomo-γ- linolenate (20:3) concentrations were unchanged. Saturated/unsaturated FFA ratio, n-3/n-6 ratio, de novo lipogenesis index (palmitate (main lipogenesis product)/linoleate (only found in diet)) and elongase index (oleate/palmitoleate) were increased in the T1DM group (p=0.0166, p=0.0089, p<0.0001 and p=0.0008 respectively). The stearoyl-CoA desaturase 1 (SCD1) index 1 (palmitoleate/palmitate) and index 2 (oleate/stearate) were reduced in T1DM (p<0.0001 for all). The delta-(5)- desaturase (D5D) index (arachidonate/dihomo-γ-linolenate) was unchanged. Age and sex had no effect on plasma FFA concentrations in T1DM, while SCD1 index 1 was positively correlated (p=0.098) and elongase index negatively correlated with age (p=0.0363). HbA1c was negatively correlated with all plasma FFAs concentrations measured except α- linolenate and dihomo-γ-linolenate. Correlations were observed between plasma FFAs and cholesterol and HDL, but not LDL or diabetes duration. Collectively, these results aid our understanding of T1DM and its effects on lipid metabolism. | |
dc.format.extent | 9 | |
dc.format.extent | 6447666 | |
dc.language.iso | eng | |
dc.relation.ispartof | Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids | en |
dc.subject | Fatty acid metabolism | en |
dc.subject | GC-MS | en |
dc.subject | HbA1c | en |
dc.subject | Lipid metabolism | en |
dc.subject | Lipidomics | en |
dc.subject | Non-esterified fatty acid | en |
dc.subject | QH301 Biology | en |
dc.subject | RC Internal medicine | en |
dc.subject | NDAS | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | RC | en |
dc.title | Lipidomic profiling of plasma free fatty acids in type-1 diabetes highlights specific changes in lipid metabolism | en |
dc.type | Journal article | en |
dc.contributor.sponsor | British Heart Foundation | en |
dc.contributor.sponsor | British Heart Foundation | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Sir James Mackenzie Institute for Early Diagnosis | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. Cellular Medicine Division | en |
dc.identifier.doi | 10.1016/j.bbalip.2020.158823 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | PG/15/9/31270 | en |
dc.identifier.grantnumber | FS/15/42/31556 | en |
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