Files in this item
Narrowband organic light-emitting diodes for fluorescence microscopy and calcium imaging
Item metadata
dc.contributor.author | Murawski, Caroline | |
dc.contributor.author | Mischok, Andreas | |
dc.contributor.author | Booth, Jonathan Hunter | |
dc.contributor.author | Kumar, Jothi Dinesh | |
dc.contributor.author | Archer, Emily | |
dc.contributor.author | Tropf, Laura Christine | |
dc.contributor.author | Keum, Changmin | |
dc.contributor.author | Deng, Yali | |
dc.contributor.author | Yoshida, Kou | |
dc.contributor.author | Samuel, Ifor David William | |
dc.contributor.author | Schubert, Marcel | |
dc.contributor.author | Pulver, Stefan | |
dc.contributor.author | Gather, Malte Christian | |
dc.date.accessioned | 2020-09-04T23:34:45Z | |
dc.date.available | 2020-09-04T23:34:45Z | |
dc.date.issued | 2019-09-05 | |
dc.identifier.citation | Murawski , C , Mischok , A , Booth , J H , Kumar , J D , Archer , E , Tropf , L C , Keum , C , Deng , Y , Yoshida , K , Samuel , I D W , Schubert , M , Pulver , S & Gather , M C 2019 , ' Narrowband organic light-emitting diodes for fluorescence microscopy and calcium imaging ' , Advanced Materials , vol. Early View . https://doi.org/10.1002/adma.201903599 | en |
dc.identifier.issn | 0935-9648 | |
dc.identifier.other | PURE: 260667582 | |
dc.identifier.other | PURE UUID: 8e7e1df1-c40a-4743-a9da-77473ad67c8a | |
dc.identifier.other | ORCID: /0000-0002-4857-5562/work/61622246 | |
dc.identifier.other | ORCID: /0000-0002-8739-4852/work/61622250 | |
dc.identifier.other | ORCID: /0000-0002-9995-6525/work/61622258 | |
dc.identifier.other | ORCID: /0000-0003-4725-7404/work/61622266 | |
dc.identifier.other | Scopus: 85071780752 | |
dc.identifier.other | ORCID: /0000-0001-5170-7522/work/69463416 | |
dc.identifier.other | WOS: 000485331100001 | |
dc.identifier.other | ORCID: /0000-0003-1086-0509/work/105957101 | |
dc.identifier.uri | https://hdl.handle.net/10023/20548 | |
dc.description | Funding: Leverhulme Trust (RPG-2017-231), the EPSRC NSF-CBET lead agency agreement (EP/R010595/1, 1706207), the DARPA NESD program (N66001-17-C-4012) and the RS Macdonald Charitable Trust. C.M. acknowledges funding from the European Commission through a Marie Skłodowska Curie individual fellowship (703387). A.M. acknowledges funding through an individual fellowship of the Deutsche Forschungsgemeinschaft (404587082). Y.D. acknowledges support from the Chinese Scholarship Council (CSC). L.T. acknowledges studentship funding through the EPSRC CM-CDT (EP/L015110/1). M.S. acknowledges funding by the Royal Society (Dorothy Hodgkin Fellowship, DH160102). | en |
dc.description.abstract | Fluorescence imaging is an indispensable tool in biology, with applications ranging from single‐cell to whole‐animal studies and with live mapping of neuronal activity currently receiving particular attention. To enable fluorescence imaging at cellular scale in freely moving animals, miniaturized microscopes and lensless imagers are developed that can be implanted in a minimally invasive fashion; but the rigidity, size, and potential toxicity of the involved light sources remain a challenge. Here, narrowband organic light‐emitting diodes (OLEDs) are developed and used for fluorescence imaging of live cells and for mapping of neuronal activity in Drosophila melanogaster via genetically encoded Ca2+ indicators. In order to avoid spectral overlap with fluorescence from the sample, distributed Bragg reflectors are integrated onto the OLEDs to block their long‐wavelength emission tail, which enables an image contrast comparable to conventional, much bulkier mercury light sources. As OLEDs can be fabricated on mechanically flexible substrates and structured into arrays of cell‐sized pixels, this work opens a new pathway for the development of implantable light sources that enable functional imaging and sensing in freely moving animals. | |
dc.format.extent | 8 | |
dc.language.iso | eng | |
dc.relation.ispartof | Advanced Materials | en |
dc.rights | Copyright © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This work has been made available online in accordance with publisher policies or with permission. Permission for further reuse of this content should be sought from the publisher or the rights holder. This is the author created accepted manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1002/adma.201903599 | en |
dc.subject | Calcium imaging | en |
dc.subject | Distributed Bragg reflector | en |
dc.subject | Fluorescence microscopy | en |
dc.subject | OLED | en |
dc.subject | QC Physics | en |
dc.subject | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry | en |
dc.subject | T Technology | en |
dc.subject | DAS | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | RC0321 | en |
dc.subject.lcc | T | en |
dc.title | Narrowband organic light-emitting diodes for fluorescence microscopy and calcium imaging | en |
dc.type | Journal article | en |
dc.contributor.sponsor | US Department of Defence | en |
dc.contributor.sponsor | European Commission | en |
dc.contributor.sponsor | The Royal Society | en |
dc.description.version | Postprint | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Psychology and Neuroscience | en |
dc.contributor.institution | University of St Andrews. Centre for Biophotonics | en |
dc.contributor.institution | University of St Andrews. Sir James Mackenzie Institute for Early Diagnosis | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. Condensed Matter Physics | en |
dc.identifier.doi | https://doi.org/10.1002/adma.201903599 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2020-09-05 | |
dc.identifier.grantnumber | N66001-17-C-4012 | en |
dc.identifier.grantnumber | 703387 | en |
dc.identifier.grantnumber | DH160102 | en |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.