Narrowband organic light-emitting diodes for fluorescence microscopy and calcium imaging
Date
05/09/2019Author
Grant ID
N66001-17-C-4012
703387
DH160102
Keywords
Metadata
Show full item recordAbstract
Fluorescence imaging is an indispensable tool in biology, with applications ranging from single‐cell to whole‐animal studies and with live mapping of neuronal activity currently receiving particular attention. To enable fluorescence imaging at cellular scale in freely moving animals, miniaturized microscopes and lensless imagers are developed that can be implanted in a minimally invasive fashion; but the rigidity, size, and potential toxicity of the involved light sources remain a challenge. Here, narrowband organic light‐emitting diodes (OLEDs) are developed and used for fluorescence imaging of live cells and for mapping of neuronal activity in Drosophila melanogaster via genetically encoded Ca2+ indicators. In order to avoid spectral overlap with fluorescence from the sample, distributed Bragg reflectors are integrated onto the OLEDs to block their long‐wavelength emission tail, which enables an image contrast comparable to conventional, much bulkier mercury light sources. As OLEDs can be fabricated on mechanically flexible substrates and structured into arrays of cell‐sized pixels, this work opens a new pathway for the development of implantable light sources that enable functional imaging and sensing in freely moving animals.
Citation
Murawski , C , Mischok , A , Booth , J H , Kumar , J D , Archer , E , Tropf , L C , Keum , C , Deng , Y , Yoshida , K , Samuel , I D W , Schubert , M , Pulver , S & Gather , M C 2019 , ' Narrowband organic light-emitting diodes for fluorescence microscopy and calcium imaging ' , Advanced Materials , vol. Early View . https://doi.org/10.1002/adma.201903599
Publication
Advanced Materials
Status
Peer reviewed
ISSN
0935-9648Type
Journal article
Description
Funding: Leverhulme Trust (RPG-2017-231), the EPSRC NSF-CBET lead agency agreement (EP/R010595/1, 1706207), the DARPA NESD program (N66001-17-C-4012) and the RS Macdonald Charitable Trust. C.M. acknowledges funding from the European Commission through a Marie Skłodowska Curie individual fellowship (703387). A.M. acknowledges funding through an individual fellowship of the Deutsche Forschungsgemeinschaft (404587082). Y.D. acknowledges support from the Chinese Scholarship Council (CSC). L.T. acknowledges studentship funding through the EPSRC CM-CDT (EP/L015110/1). M.S. acknowledges funding by the Royal Society (Dorothy Hodgkin Fellowship, DH160102).Collections
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