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dc.contributor.authorZhang, Xing
dc.contributor.authorCampbell, Raymond
dc.contributor.authorDucreux, Laurence J. M.
dc.contributor.authorMorris, Jennifer
dc.contributor.authorHedley, Pete E
dc.contributor.authorMellado-Ortega, Elena
dc.contributor.authorRoberts, Alison G
dc.contributor.authorStephens, Jennifer
dc.contributor.authorBryan, Glenn J
dc.contributor.authorTorrance, Lesley
dc.contributor.authorChapman, Sean N
dc.contributor.authorPrat, Salomé
dc.contributor.authorTaylor, Mark A
dc.identifier.citationZhang , X , Campbell , R , Ducreux , L J M , Morris , J , Hedley , P E , Mellado-Ortega , E , Roberts , A G , Stephens , J , Bryan , G J , Torrance , L , Chapman , S N , Prat , S & Taylor , M A 2020 , ' TERMINAL FLOWER-1/CENTRORADIALIS inhibits tuberization via protein interaction with the tuberigen activation complex ' , The Plant Journal , vol. Early View .
dc.identifier.otherPURE: 268762602
dc.identifier.otherPURE UUID: a28e9485-1ecd-416a-be4f-962a7581597f
dc.identifier.otherPubMed: 32593210
dc.identifier.otherWOS: 000548226800001
dc.identifier.otherScopus: 85087896753
dc.descriptionThis work was funded by the Scottish Government Rural and Environment Science and Analytical Services Division as part of the Strategic Research Programme 2016-2021, by a GCRF Foundation Awards for Global Agricultural and Food Systems Research funded by the BBSRC project BB/P022553/1 and also received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement number 835704. Research in Prat’s lab was funded by the Spanish Ministerio de Economía y Competitividad BIO2015-73019-EXP, and the aligned Japan EIG CONCERT (PIA102017-1) projects.en
dc.description.abstractPotato tuber formation is a secondary developmental program by which cells in the subapical stolon region divide and radially expand, to further differentiate into starch accumulating parenchyma. Whilst some details of the molecular pathway that signals tuberization are known, important gaps in our knowledge persist. Here the role of a member of the TERMINAL FLOWER 1/ CENTRORADIALIS gene family (termed StCEN ) in the negative control of tuberization is demonstrated for the first time. It is shown that reduced expression of StCEN accelerates tuber formation whereas transgenic lines over‐expressing this gene display delayed tuberization and reduced tuber yield. Protein‐protein interaction studies (yeast two hybrid and bimolecular fluorescence complementation) demonstrate that StCEN binds components of the recently described tuberigen activation complex. Using transient transactivation assays we show that the StSP6A tuberization signal is an activation target of the tuberigen activation complex, and that co‐expression of StCEN blocks StFD‐Like‐1 activation of the StSP6A gene. Transcriptomic analysis of transgenic lines mis‐expressing StCEN identify early transcriptional events in tuber formation. These results demonstrate that StCEN suppresses tuberization by directly antagonizing StSP6A function in stolons, identifying StCEN as a breeding marker to improve tuber initiation and yield, through the selection of genotypes with reduced StCEN expression.
dc.relation.ispartofThe Plant Journalen
dc.rightsCopyright © 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.subjectProtein-protein interactionen
dc.subjectSolanum tuberosum (potato)en
dc.subjectTuberigen complexen
dc.subjectQH301 Biologyen
dc.titleTERMINAL FLOWER-1/CENTRORADIALIS inhibits tuberization via protein interaction with the tuberigen activation complexen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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