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TERMINAL FLOWER-1/CENTRORADIALIS inhibits tuberization via protein interaction with the tuberigen activation complex

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Zhang_20250_TERMINAl_FLOWER_1_PlantJ_CC.pdf (773.4Kb)
Date
14/07/2020
Author
Zhang, Xing
Campbell, Raymond
Ducreux, Laurence J. M.
Morris, Jennifer
Hedley, Pete E
Mellado-Ortega, Elena
Roberts, Alison G
Stephens, Jennifer
Bryan, Glenn J
Torrance, Lesley
Chapman, Sean N
Prat, Salomé
Taylor, Mark A
Keywords
Protein-protein interaction
Solanum tuberosum (potato)
TERMINAL FLOWER 1/CENTRORADIALIS
Tuberigen complex
Tuberisation
QH301 Biology
DAS
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Abstract
Potato tuber formation is a secondary developmental program by which cells in the subapical stolon region divide and radially expand, to further differentiate into starch accumulating parenchyma. Whilst some details of the molecular pathway that signals tuberization are known, important gaps in our knowledge persist. Here the role of a member of the TERMINAL FLOWER 1/ CENTRORADIALIS gene family (termed StCEN ) in the negative control of tuberization is demonstrated for the first time. It is shown that reduced expression of StCEN accelerates tuber formation whereas transgenic lines over‐expressing this gene display delayed tuberization and reduced tuber yield. Protein‐protein interaction studies (yeast two hybrid and bimolecular fluorescence complementation) demonstrate that StCEN binds components of the recently described tuberigen activation complex. Using transient transactivation assays we show that the StSP6A tuberization signal is an activation target of the tuberigen activation complex, and that co‐expression of StCEN blocks StFD‐Like‐1 activation of the StSP6A gene. Transcriptomic analysis of transgenic lines mis‐expressing StCEN identify early transcriptional events in tuber formation. These results demonstrate that StCEN suppresses tuberization by directly antagonizing StSP6A function in stolons, identifying StCEN as a breeding marker to improve tuber initiation and yield, through the selection of genotypes with reduced StCEN expression.
Citation
Zhang , X , Campbell , R , Ducreux , L J M , Morris , J , Hedley , P E , Mellado-Ortega , E , Roberts , A G , Stephens , J , Bryan , G J , Torrance , L , Chapman , S N , Prat , S & Taylor , M A 2020 , ' TERMINAL FLOWER-1/CENTRORADIALIS inhibits tuberization via protein interaction with the tuberigen activation complex ' , The Plant Journal , vol. Early View . https://doi.org/10.1111/tpj.14898
Publication
The Plant Journal
Status
Peer reviewed
DOI
https://doi.org/10.1111/tpj.14898
ISSN
0960-7412
Type
Journal article
Rights
Copyright © 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Description
This work was funded by the Scottish Government Rural and Environment Science and Analytical Services Division as part of the Strategic Research Programme 2016-2021, by a GCRF Foundation Awards for Global Agricultural and Food Systems Research funded by the BBSRC project BB/P022553/1 and also received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement number 835704. Research in Prat’s lab was funded by the Spanish Ministerio de Economía y Competitividad BIO2015-73019-EXP, and the aligned Japan EIG CONCERT (PIA102017-1) projects.
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/20292

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