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Integrated holographic system for all-optical manipulation of developing embryos
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dc.contributor.author | Torres, Maria Leilani | |
dc.contributor.author | Antkowiak, Maciej | |
dc.contributor.author | Cizmarova, Hana | |
dc.contributor.author | Ferrier, David Ellard Keith | |
dc.contributor.author | Dholakia, Kishan | |
dc.contributor.author | Gunn-Moore, Frank J | |
dc.date.accessioned | 2011-09-26T11:26:39Z | |
dc.date.available | 2011-09-26T11:26:39Z | |
dc.date.issued | 2011-06-01 | |
dc.identifier | 8464283 | |
dc.identifier | cfc8660b-63ef-496d-b196-47637e0b9dba | |
dc.identifier | 84861920133 | |
dc.identifier | 000299878800016 | |
dc.identifier.citation | Torres , M L , Antkowiak , M , Cizmarova , H , Ferrier , D E K , Dholakia , K & Gunn-Moore , F J 2011 , ' Integrated holographic system for all-optical manipulation of developing embryos ' , Biomedical Optics Express , vol. 2 , no. 6 , pp. 1564-1575 . https://doi.org/10.1364/BOE.2.001564 | en |
dc.identifier.issn | 2156-7085 | |
dc.identifier.other | ORCID: /0000-0003-3247-6233/work/36423830 | |
dc.identifier.other | ORCID: /0000-0003-3422-3387/work/34730451 | |
dc.identifier.uri | https://hdl.handle.net/10023/2007 | |
dc.description.abstract | We demonstrate a system for the combined optical injection and trapping of developing embryos. A Ti:sapphire femtosecond laser in tandem with a spatial light modulator, is used to perform fast and accurate beam-steering and multiplexing. We show successful intracellular delivery of a range of impermeable molecules into individual blastomeres of the annelid Pomatoceros lamarckii embryo by optoinjection, even when the embryo is still enclosed in a chorion. We also demonstrate the ability of the femtosecond laser optoinjection to deliver materials into inner layers of cells in a well-developed embryo. By switching to the continuous wave mode of the Ti:sapphire laser, the same system can be employed to optically trap and orient the 60 μm sized P. lamarckii embryo whilst maintaining its viability. Hence, a complete all-optical manipulation platform is demonstrated paving the way towards single-cell genetic modification and cell lineage mapping in emerging developmental biology model species. | |
dc.format.extent | 11 | |
dc.format.extent | 1188195 | |
dc.language.iso | eng | |
dc.relation.ispartof | Biomedical Optics Express | en |
dc.subject | Multiphoton processes | en |
dc.subject | Lasers | en |
dc.subject | Ultrafast lasers | en |
dc.subject | QC Physics | en |
dc.subject.lcc | QC | en |
dc.title | Integrated holographic system for all-optical manipulation of developing embryos | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. Scottish Oceans Institute | en |
dc.contributor.institution | University of St Andrews. Marine Alliance for Science & Technology Scotland | en |
dc.identifier.doi | 10.1364/BOE.2.001564 | |
dc.description.status | Peer reviewed | en |
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