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dc.contributor.authorTorres, Maria Leilani
dc.contributor.authorAntkowiak, Maciej
dc.contributor.authorCizmarova, Hana
dc.contributor.authorFerrier, David Ellard Keith
dc.contributor.authorDholakia, Kishan
dc.contributor.authorGunn-Moore, Frank J
dc.identifier.citationTorres , M L , Antkowiak , M , Cizmarova , H , Ferrier , D E K , Dholakia , K & Gunn-Moore , F J 2011 , ' Integrated holographic system for all-optical manipulation of developing embryos ' , Biomedical Optics Express , vol. 2 , no. 6 , pp. 1564-1575 .
dc.identifier.otherPURE: 8464283
dc.identifier.otherPURE UUID: cfc8660b-63ef-496d-b196-47637e0b9dba
dc.identifier.otherScopus: 84861920133
dc.identifier.otherWOS: 000299878800016
dc.identifier.otherORCID: /0000-0003-3247-6233/work/36423830
dc.identifier.otherORCID: /0000-0003-3422-3387/work/34730451
dc.description.abstractWe demonstrate a system for the combined optical injection and trapping of developing embryos. A Ti:sapphire femtosecond laser in tandem with a spatial light modulator, is used to perform fast and accurate beam-steering and multiplexing. We show successful intracellular delivery of a range of impermeable molecules into individual blastomeres of the annelid Pomatoceros lamarckii embryo by optoinjection, even when the embryo is still enclosed in a chorion. We also demonstrate the ability of the femtosecond laser optoinjection to deliver materials into inner layers of cells in a well-developed embryo. By switching to the continuous wave mode of the Ti:sapphire laser, the same system can be employed to optically trap and orient the 60 μm sized P. lamarckii embryo whilst maintaining its viability. Hence, a complete all-optical manipulation platform is demonstrated paving the way towards single-cell genetic modification and cell lineage mapping in emerging developmental biology model species.
dc.relation.ispartofBiomedical Optics Expressen
dc.rights©2011 Optical Society of America. This paper was published in Biomedical Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.en
dc.subjectMultiphoton processesen
dc.subjectUltrafast lasersen
dc.subjectQC Physicsen
dc.titleIntegrated holographic system for all-optical manipulation of developing embryosen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Scottish Oceans Instituteen
dc.contributor.institutionUniversity of St Andrews. Marine Alliance for Science & Technology Scotlanden
dc.description.statusPeer revieweden

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