Integrated holographic system for all-optical manipulation of developing embryos
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We demonstrate a system for the combined optical injection and trapping of developing embryos. A Ti:sapphire femtosecond laser in tandem with a spatial light modulator, is used to perform fast and accurate beam-steering and multiplexing. We show successful intracellular delivery of a range of impermeable molecules into individual blastomeres of the annelid Pomatoceros lamarckii embryo by optoinjection, even when the embryo is still enclosed in a chorion. We also demonstrate the ability of the femtosecond laser optoinjection to deliver materials into inner layers of cells in a well-developed embryo. By switching to the continuous wave mode of the Ti:sapphire laser, the same system can be employed to optically trap and orient the 60 μm sized P. lamarckii embryo whilst maintaining its viability. Hence, a complete all-optical manipulation platform is demonstrated paving the way towards single-cell genetic modification and cell lineage mapping in emerging developmental biology model species.
Torres , M L , Antkowiak , M , Cizmarova , H , Ferrier , D E K , Dholakia , K & Gunn-Moore , F J 2011 , ' Integrated holographic system for all-optical manipulation of developing embryos ' Biomedical Optics Express , vol. 2 , no. 6 , pp. 1564-1575 . DOI: 10.1364/BOE.2.001564
Biomedical Optics Express
©2011 Optical Society of America. This paper was published in Biomedical Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/abstract.cfm?URI=boe-2-6-1564. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
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