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dc.contributor.authorPulido Companys, Pau
dc.contributor.authorNorris, Anneliese
dc.contributor.authorBischoff, Marcus
dc.date.accessioned2020-03-31T12:30:03Z
dc.date.available2020-03-31T12:30:03Z
dc.date.issued2020-03-30
dc.identifier.citationPulido Companys , P , Norris , A & Bischoff , M 2020 , ' Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis ' , Journal of Cell Science , vol. 133 , no. 6 , jcs235325 . https://doi.org/10.1242/jcs.235325en
dc.identifier.issn0021-9533
dc.identifier.otherPURE: 265898135
dc.identifier.otherPURE UUID: b5d00b3b-b00f-43de-9af2-301d31e5d01c
dc.identifier.otherORCID: /0000-0002-0737-6321/work/71559951
dc.identifier.otherWOS: 000534387000009
dc.identifier.otherScopus: 85082791675
dc.identifier.urihttp://hdl.handle.net/10023/19733
dc.descriptionThis work was supported by the BBSRC (BB/M021084/1).en
dc.description.abstractDuring morphogenesis, cells exhibit various behaviours, such as migration and constriction, which need to be coordinated. How this is achieved remains elusive. During morphogenesis of the Drosophila adult abdominal epidermis, larval epithelial cells (LECs) migrate directedly before constricting apically and undergoing apoptosis. Here, we study the mechanisms underlying the transition from migration to constriction. We show that LECs possess a pulsatile apical actomyosin network, and that a change in network polarity correlates with behavioural change. Exploring the properties of the contractile network, we find that cell contractility, as determined by myosin activity, has an impact on the behaviour of the network, as well as on cytoskeletal architecture and cell behaviour. Pulsed contractions occur only in cells with intermediate levels of contractility. Furthermore, increasing levels of the small Rho GTPase Rho1 disrupts pulsing, leading to cells that cycle between two states, characterised by a junctional cortical and an apicomedial actin network. Our results highlight that behavioural change relies on tightly controlled cellular contractility. Moreover, we show that constriction can occur without pulsing, raising questions why constricting cells pulse in some contexts but not in others.
dc.format.extent18
dc.language.isoeng
dc.relation.ispartofJournal of Cell Scienceen
dc.rightsCopyright © 2020 The Author(s). Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.en
dc.subjectCell migrationen
dc.subjectApical constrictionen
dc.subjectPulsed contractionsen
dc.subjectActomyosin contractilityen
dc.subjectDrosophilaen
dc.subjectQH301 Biologyen
dc.subjectDASen
dc.subject.lccQH301en
dc.titleCoordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesisen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.identifier.doihttps://doi.org/10.1242/jcs.235325
dc.description.statusPeer revieweden
dc.date.embargoedUntil2020-03-30


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