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dc.contributor.authorNascimento, Filipe
dc.contributor.authorBroadhead, Matthew James
dc.contributor.authorTetringa, Efstathia
dc.contributor.authorTsape, Eirini
dc.contributor.authorZagoraiou, Laskaro
dc.contributor.authorMiles, Gareth Brian
dc.identifier.citationNascimento , F , Broadhead , M J , Tetringa , E , Tsape , E , Zagoraiou , L & Miles , G B 2020 , ' Synaptic mechanisms underlying modulation of locomotor-related motoneuron output by premotor cholinergic interneurons ' , eLife , vol. 9 , e54170 .
dc.identifier.otherPURE: 266483367
dc.identifier.otherPURE UUID: 3daee16f-c5a8-4e88-88ed-44c4af972939
dc.identifier.otherORCID: /0000-0002-8624-4625/work/70618975
dc.identifier.otherScopus: 85081944744
dc.identifier.otherWOS: 000519713500001
dc.descriptionF Nascimento was supported by The Alfred Dunhill Links Foundation. G B Miles and M J Broadhead received support from Biotechnology and Biological Sciences Research Council Grant BB/M021793/1. L Zagoraiou and E Tsape were supported by Fondation Santé.en
dc.description.abstractSpinal motor networks are formed by diverse populations of interneurons that set the strength and rhythmicity of behaviors such as locomotion. A small cluster of cholinergic interneurons, expressing the transcription factor Pitx2, modulates the intensity of muscle activation via ‘C-bouton’ inputs to motoneurons. However, the synaptic mechanisms underlying this neuromodulation remain unclear. Here, we confirm in mice that Pitx2+ interneurons are active during fictive locomotion and that their chemogenetic inhibition reduces the amplitude of motor output. Furthermore, after genetic ablation of cholinergic Pitx2+ interneurons, M2 receptor-dependent regulation of the intensity of locomotor output is lost. Conversely, chemogenetic stimulation of Pitx2+ interneurons leads to activation of M2 receptors on motoneurons, regulation of Kv2.1 channels and greater motoneuron output due to an increase in the inter-spike afterhyperpolarization and a reduction in spike half-width. Our findings elucidate synaptic mechanisms by which cholinergic spinal interneurons modulate the final common pathway for motor output.
dc.rightsCopyright © 2020, Nascimento et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.en
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.titleSynaptic mechanisms underlying modulation of locomotor-related motoneuron output by premotor cholinergic interneuronsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Psychology and Neuroscienceen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.description.statusPeer revieweden

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