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Synaptic mechanisms underlying modulation of locomotor-related motoneuron output by premotor cholinergic interneurons

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Nascimento_2020_eLife_Synaptic_CC.pdf (3.412Mb)
Date
09/03/2020
Author
Nascimento, Filipe
Broadhead, Matthew James
Tetringa, Efstathia
Tsape, Eirini
Zagoraiou, Laskaro
Miles, Gareth Brian
Funder
BBSRC
BBSRC
Grant ID
BB/M021793/1
BB/E019803/1
Keywords
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
DAS
BDC
R2C
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Abstract
Spinal motor networks are formed by diverse populations of interneurons that set the strength and rhythmicity of behaviors such as locomotion. A small cluster of cholinergic interneurons, expressing the transcription factor Pitx2, modulates the intensity of muscle activation via ‘C-bouton’ inputs to motoneurons. However, the synaptic mechanisms underlying this neuromodulation remain unclear. Here, we confirm in mice that Pitx2+ interneurons are active during fictive locomotion and that their chemogenetic inhibition reduces the amplitude of motor output. Furthermore, after genetic ablation of cholinergic Pitx2+ interneurons, M2 receptor-dependent regulation of the intensity of locomotor output is lost. Conversely, chemogenetic stimulation of Pitx2+ interneurons leads to activation of M2 receptors on motoneurons, regulation of Kv2.1 channels and greater motoneuron output due to an increase in the inter-spike afterhyperpolarization and a reduction in spike half-width. Our findings elucidate synaptic mechanisms by which cholinergic spinal interneurons modulate the final common pathway for motor output.
Citation
Nascimento , F , Broadhead , M J , Tetringa , E , Tsape , E , Zagoraiou , L & Miles , G B 2020 , ' Synaptic mechanisms underlying modulation of locomotor-related motoneuron output by premotor cholinergic interneurons ' , eLife , vol. 9 , e54170 . https://doi.org/10.7554/eLife.54170
Publication
eLife
Status
Peer reviewed
DOI
https://doi.org/10.7554/eLife.54170
ISSN
2050-084X
Type
Journal article
Rights
Copyright © 2020, Nascimento et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Description
F Nascimento was supported by The Alfred Dunhill Links Foundation. G B Miles and M J Broadhead received support from Biotechnology and Biological Sciences Research Council Grant BB/M021793/1. L Zagoraiou and E Tsape were supported by Fondation Santé.
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/19633

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