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dc.contributor.authorWang, Kimberley C.W.
dc.contributor.authorAstell, Chrissie J.
dc.contributor.authorWijesinghe, Philip
dc.contributor.authorLarcombe, Alexander N.
dc.contributor.authorPinniger, Gavin J.
dc.contributor.authorZosky, Graeme R.
dc.contributor.authorKennedy, Brendan F.
dc.contributor.authorBerry, Luke J.
dc.contributor.authorSampson, David D.
dc.contributor.authorJames, Alan L.
dc.contributor.authorLe Cras, Timothy D.
dc.contributor.authorNoble, Peter B.
dc.identifier.citationWang , K C W , Astell , C J , Wijesinghe , P , Larcombe , A N , Pinniger , G J , Zosky , G R , Kennedy , B F , Berry , L J , Sampson , D D , James , A L , Le Cras , T D & Noble , P B 2017 , ' Optical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease ' , Scientific Reports , vol. 7 , 1517 .
dc.identifier.otherPURE: 266562745
dc.identifier.otherPURE UUID: 7609e134-8fc0-4e72-a6c3-3a7a5dc6985a
dc.identifier.otherScopus: 85018426604
dc.identifier.otherPubMed: 28473708
dc.identifier.otherORCID: /0000-0002-8378-7261/work/69835220
dc.descriptionFunding provided by the National Health and Medical Research Council (NHMRC) of Australia (1027218). P.N. and K.W. are supported by NHMRC Fellowships (1045824, 1090888). P.W. was supported by the William and Marlene Schrader Postgraduate Scholarship, The University of Western Australia, and C.A. by an NHMRC Preterm Infants CRE top-up scholarship.en
dc.description.abstractThis study tested the utility of optical coherence tomography (OCT)-based indentation to assess mechanical properties of respiratory tissues in disease. Using OCT-based indentation, the elastic modulus of mouse diaphragm was measured from changes in diaphragm thickness in response to an applied force provided by an indenter. We used a transgenic mouse model of chronic lung disease induced by the overexpression of transforming growth factor-alpha (TGF-α), established by the presence of pleural and peribronchial fibrosis and impaired lung mechanics determined by the forced oscillation technique and plethysmography. Diaphragm elastic modulus assessed by OCT-based indentation was reduced by TGF-α at both left and right lateral locations (p < 0.05). Diaphragm elastic modulus at left and right lateral locations were correlated within mice (r = 0.67, p < 0.01) suggesting that measurements were representative of tissue beyond the indenter field. Co-localised images of diaphragm after TGF-α overexpression revealed a layered fibrotic appearance. Maximum diaphragm force in conventional organ bath studies was also reduced by TGF-α overexpression (p < 0.01). Results show that OCT-based indentation provided clear delineation of diseased diaphragm, and together with organ bath assessment, provides new evidence suggesting that TGF-α overexpression produces impairment in diaphragm function and, therefore, an increase in the work of breathing in chronic lung disease.
dc.relation.ispartofScientific Reportsen
dc.rightsCopyright © The Author(s) 2017. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
dc.subjectOptical imagingen
dc.subjectQC Physicsen
dc.subjectQH301 Biologyen
dc.subjectRC Internal medicineen
dc.titleOptical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung diseaseen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.description.statusPeer revieweden

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