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Three-dimensional imaging of cell and extracellular matrix elasticity using quantitative micro-elastography

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Wijesinghe_2020_BOE_three_dim_867.pdf (5.914Mb)
Date
01/02/2020
Author
Hepburn, Matt S.
Wijesinghe, Philip
Major, Luke G.
Li, Jiayue
Mowla, Alireza
Astell, Chrissie
Park, Hyun Woo
Hwang, Yongsung
Choi, Yu Suk
Kennedy, Brendan F.
Keywords
QC Physics
QH301 Biology
Biotechnology
Atomic and Molecular Physics, and Optics
NDAS
Metadata
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Abstract
Recent studies in mechanobiology have revealed the importance of cellular and extracellular mechanical properties in regulating cellular function in normal and disease states. Although it is established that cells should be investigated in a three-dimensional (3-D) environment, most techniques available to study mechanical properties on the microscopic scale are unable to do so. In this study, for the first time, we present volumetric images of cellular and extracellular elasticity in 3-D biomaterials using quantitative micro-elastography (QME). We achieve this by developing a novel strain estimation algorithm based on 3-D linear regression to improve QME system resolution. We show that QME can reveal elevated elasticity surrounding human adipose-derived stem cells (ASCs) embedded in soft hydrogels. We observe, for the first time in 3-D, further elevation of extracellular elasticity around ASCs with overexpressed TAZ; a mechanosensitive transcription factor which regulates cell volume. Our results demonstrate that QME has the potential to study the effects of extracellular mechanical properties on cellular functions in a 3-D micro-environment.
Citation
Hepburn , M S , Wijesinghe , P , Major , L G , Li , J , Mowla , A , Astell , C , Park , H W , Hwang , Y , Choi , Y S & Kennedy , B F 2020 , ' Three-dimensional imaging of cell and extracellular matrix elasticity using quantitative micro-elastography ' , Biomedical Optics Express , vol. 11 , no. 2 , pp. 867-884 . https://doi.org/10.1364/BOE.383419
Publication
Biomedical Optics Express
Status
Peer reviewed
DOI
https://doi.org/10.1364/BOE.383419
ISSN
2156-7085
Type
Journal article
Rights
Copright © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Description
Funding: Australian Research Council; Cancer Council Western Australia; Industrial Transformation Training Centre; The William and Marlene Schrader Trust of the University of Western Australia.
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/19521

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