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2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis
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dc.contributor.author | Hardwick, Jack | |
dc.contributor.author | Haugland, Marius | |
dc.contributor.author | El-Sagheer, Afaf | |
dc.contributor.author | Ptchelkine, Denis | |
dc.contributor.author | Beierlein, Frank | |
dc.contributor.author | Lane, Andrew | |
dc.contributor.author | Brown, Tom | |
dc.contributor.author | Lovett, Janet E. | |
dc.contributor.author | Anderson, Edward | |
dc.date.accessioned | 2020-02-13T16:36:02Z | |
dc.date.available | 2020-02-13T16:36:02Z | |
dc.date.issued | 2020-02-13 | |
dc.identifier.citation | Hardwick , J , Haugland , M , El-Sagheer , A , Ptchelkine , D , Beierlein , F , Lane , A , Brown , T , Lovett , J E & Anderson , E 2020 , ' 2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis ' , Nucleic Acids Research , vol. Advance articles , gkaa086 . https://doi.org/10.1093/nar/gkaa086 | en |
dc.identifier.issn | 0305-1048 | |
dc.identifier.other | PURE: 266318227 | |
dc.identifier.other | PURE UUID: eeedd476-95cb-4a1e-86f6-bc13c0f4c158 | |
dc.identifier.other | ORCID: /0000-0002-3561-450X/work/69029311 | |
dc.identifier.other | Scopus: 85082560953 | |
dc.identifier.other | WOS: 000525957500009 | |
dc.identifier.uri | https://hdl.handle.net/10023/19457 | |
dc.description | Funding: Engineering and Physical Sciences Research Council [EP/M019195/1]; Engineering and Physical Sciences Research Council Studentship (to J.S.H.); Biotechnology and Biological Sciences Research Council [BB/J001694/2, BB/R021848/1]; ADTBio; University of Kentucky and NCI Cancer Center Support Grant [P30 CA177558]; The Carmen L. Buck Endowment; Emerging Fields Initiative of the Friedrich-Alexander-University of Erlangen-Nuremberg [Grant title ‘Chemistry in Live Cells’]; Wellcome Trust [099149/Z/12/Z]; Royal Society, University Research Fellowship (to J.E.L.). Funding for open access charge: University of Oxford. | en |
dc.description.abstract | The determination of distances between specific points in nucleic acids is essential to understanding their behaviour at the molecular level. The ability to measure distances of 2–10 nm is particularly important: deformations arising from protein binding commonly fall within this range, but the reliable measurement of such distances for a conformational ensemble remains a significant challenge. Using several techniques, we show that electron paramagnetic resonance (EPR) spectroscopy of oligonucleotides spin-labelled with triazole-appended nitroxides at the 2′ position offers a robust and minimally perturbing tool for obtaining such measurements. For two nitroxides, we present results from EPR spectroscopy, X-ray crystal structures of B-form spin-labelled DNA duplexes, molecular dynamics simulations and nuclear magnetic resonance spectroscopy. These four methods are mutually supportive, and pinpoint the locations of the spin labels on the duplexes. In doing so, this work establishes 2′-alkynyl nitroxide spin-labelling as a minimally perturbing method for probing DNA conformation. | |
dc.language.iso | eng | |
dc.relation.ispartof | Nucleic Acids Research | en |
dc.rights | Copyright © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | en |
dc.subject | QD Chemistry | en |
dc.subject | DAS | en |
dc.subject.lcc | QD | en |
dc.title | 2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis | en |
dc.type | Journal article | en |
dc.contributor.sponsor | BBSRC | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.contributor.sponsor | The Royal Society | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.identifier.doi | https://doi.org/10.1093/nar/gkaa086 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | BB/R021848/1 | en |
dc.identifier.grantnumber | 099149/Z/12/Z | en |
dc.identifier.grantnumber | UF150698 | en |
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