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2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis

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Hardwick_2020_NAR_2_alkynyl_CC.pdf (3.623Mb)
Date
13/02/2020
Author
Hardwick, Jack
Haugland, Marius
El-Sagheer, Afaf
Ptchelkine, Denis
Beierlein, Frank
Lane, Andrew
Brown, Tom
Lovett, Janet E.
Anderson, Edward
Funder
BBSRC
The Wellcome Trust
The Royal Society
Grant ID
BB/R021848/1
099149/Z/12/Z
UF150698
Keywords
QD Chemistry
DAS
Metadata
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Abstract
The determination of distances between specific points in nucleic acids is essential to understanding their behaviour at the molecular level. The ability to measure distances of 2–10 nm is particularly important: deformations arising from protein binding commonly fall within this range, but the reliable measurement of such distances for a conformational ensemble remains a significant challenge. Using several techniques, we show that electron paramagnetic resonance (EPR) spectroscopy of oligonucleotides spin-labelled with triazole-appended nitroxides at the 2′ position offers a robust and minimally perturbing tool for obtaining such measurements. For two nitroxides, we present results from EPR spectroscopy, X-ray crystal structures of B-form spin-labelled DNA duplexes, molecular dynamics simulations and nuclear magnetic resonance spectroscopy. These four methods are mutually supportive, and pinpoint the locations of the spin labels on the duplexes. In doing so, this work establishes 2′-alkynyl nitroxide spin-labelling as a minimally perturbing method for probing DNA conformation.
Citation
Hardwick , J , Haugland , M , El-Sagheer , A , Ptchelkine , D , Beierlein , F , Lane , A , Brown , T , Lovett , J E & Anderson , E 2020 , ' 2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis ' , Nucleic Acids Research , vol. Advance articles , gkaa086 . https://doi.org/10.1093/nar/gkaa086
Publication
Nucleic Acids Research
Status
Peer reviewed
DOI
https://doi.org/10.1093/nar/gkaa086
ISSN
0305-1048
Type
Journal article
Rights
Copyright © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Description
Funding: Engineering and Physical Sciences Research Council [EP/M019195/1]; Engineering and Physical Sciences Research Council Studentship (to J.S.H.); Biotechnology and Biological Sciences Research Council [BB/J001694/2, BB/R021848/1]; ADTBio; University of Kentucky and NCI Cancer Center Support Grant [P30 CA177558]; The Carmen L. Buck Endowment; Emerging Fields Initiative of the Friedrich-Alexander-University of Erlangen-Nuremberg [Grant title ‘Chemistry in Live Cells’]; Wellcome Trust [099149/Z/12/Z]; Royal Society, University Research Fellowship (to J.E.L.). Funding for open access charge: University of Oxford.
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/19457

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