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dc.contributor.authorNdhlovu, Victor
dc.contributor.authorKiran, Anmol
dc.contributor.authorSloan, Derek
dc.contributor.authorMandala, Wilson
dc.contributor.authorKontogianni, Konstantina
dc.contributor.authorKamdolozi, Mercy
dc.contributor.authorCaws, Maxine
dc.contributor.authorDavies, Gerry
dc.identifier.citationNdhlovu , V , Kiran , A , Sloan , D , Mandala , W , Kontogianni , K , Kamdolozi , M , Caws , M & Davies , G 2019 , ' Genetic diversity of Mycobacterium tuberculosis clinical isolates in Blantyre, Malawi ' , Heliyon , vol. 5 , no. 10 , e02638 .
dc.identifier.otherPURE: 262151343
dc.identifier.otherPURE UUID: 50e51096-c609-40f1-8064-00bf37585275
dc.identifier.otherScopus: 85072967420
dc.identifier.otherWOS: 000494641300101
dc.descriptionThis work was supported by The Helse Nord Tuberculosis Initiative (HNTI).en
dc.description.abstractDespite the high burden of tuberculosis (TB) worldwide, specific factors influencing disease transmission remain elusive. Long term epidemiological studies and in vitro experimental models provide evidence of variable relative fitness of Mycobacterium tuberculosis (Mtb) strains but few such studies are available. Large sequence polymorphisms (LSP) are a robust molecular marker and are feasible as an epidemiological investigative tool. Few Mtb molecular epidemiological studies have been reported in Malawi owing to lack of laboratories with molecular tools. We characterized the genetic diversity of Mtb clinical isolates amongst TB patients in Blantyre, Malawi. We genotyped 64 Mtb clinical isolates using LSP-PCR, assigned specific lineages and confirmed 18 of the isolates using SMRT sequencing. The 64 isolates clustered into 4 lineages (L1-L4) with L4 predominating. There were 10/64 (16%) isolates belonging to L1, 6/64 (9%) belonging to L2, 2/64 (3%) belonging to L3 and 46/64 (72%) belonging to L4. Comparison with a previous study done in Karonga revealed concordance in L1 and L4 but discodance in L2 and L3. The phylogenetic tree constructed, comprised of 3/4 lineages present in Blantyre with 3/18 belonging to L1, 3/18 belonging to L2 and 12/18 belonging to L4. Four Mtb lineages were present in Blantyre with L4 predominating. Larger studies are needed to understand the molecular epidemiology of TB in Blantyre in light of increased bi-directional migration with South Africa.
dc.rightsCopyright © 2019 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (
dc.subjectClinical geneticsen
dc.subjectInfectious diseaseen
dc.subjectLarge sequence polymorphismsen
dc.subjectMycobacterium tuberculosisen
dc.subjectRegion of differenceen
dc.subjectSingle molecule real time sequencingen
dc.subjectSingle nucleotide polymorphismen
dc.subjectQR Microbiologyen
dc.subjectQH426 Geneticsen
dc.titleGenetic diversity of Mycobacterium tuberculosis clinical isolates in Blantyre, Malawien
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Infection and Global Health Divisionen
dc.description.statusPeer revieweden

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