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The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay

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dc.contributor.authorRachow, Andrea
dc.contributor.authorSaathoff, Elmar
dc.contributor.authorMtafya, Bariki
dc.contributor.authorMapamba, Daniel
dc.contributor.authorMangu, Chacha
dc.contributor.authorRojas-Ponce, Gabriel
dc.contributor.authorNtinginya, Nyanda E.
dc.contributor.authorBoeree, Martin
dc.contributor.authorHeinrich, Norbert
dc.contributor.authorGillespie, Stephen H.
dc.contributor.authorHoelscher, Michael
dc.contributor.authorPanACEA Consortium
dc.date.accessioned2019-04-03T23:37:51Z
dc.date.available2019-04-03T23:37:51Z
dc.date.issued2018-04-04
dc.identifier252751411
dc.identifier76d5bd00-5b24-490a-8877-669e6a9f5431
dc.identifier85055635262
dc.identifier000432585300010
dc.identifier.citationRachow , A , Saathoff , E , Mtafya , B , Mapamba , D , Mangu , C , Rojas-Ponce , G , Ntinginya , N E , Boeree , M , Heinrich , N , Gillespie , S H , Hoelscher , M & PanACEA Consortium 2018 , ' The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay ' , Tuberculosis , vol. In press . https://doi.org/10.1016/j.tube.2018.04.001en
dc.identifier.issn1472-9792
dc.identifier.otherRIS: urn:58D0AB99A02589C6B489C1CAD32B717B
dc.identifier.otherORCID: /0000-0001-6537-7712/work/43387998
dc.identifier.urihttps://hdl.handle.net/10023/17435
dc.descriptionThe study was supported by the German Ministry of Education and Research (BMBF, Grant No. 01KA0901 ) and the European and Developing Countries Clinical Trials Partnership (EDCTP, Project-ID: IP.2007.32011.011 ).en
dc.description.abstractThe Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs. Our data indicate that NALC/NaOH-decontamination has an effect on the performance of the Xpert assay, and that CTmin readouts of decontaminated sputum samples should be interpreted with caution.
dc.format.extent682836
dc.language.isoeng
dc.relation.ispartofTuberculosisen
dc.subjectXpert-inhibitionen
dc.subjectSputum processingen
dc.subjectQuantitative PCRen
dc.subjectXpert-performanceen
dc.subjectRA0421 Public health. Hygiene. Preventive Medicineen
dc.subjectRZ Other systems of medicineen
dc.subjectNDASen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subject.lccRA0421en
dc.subject.lccRZen
dc.titleThe impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assayen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. Global Health Implementation Groupen
dc.contributor.institutionUniversity of St Andrews. Gillespie Groupen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.identifier.doi10.1016/j.tube.2018.04.001
dc.description.statusPeer revieweden
dc.date.embargoedUntil2019-04-04


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