The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay
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The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs. Our data indicate that NALC/NaOH-decontamination has an effect on the performance of the Xpert assay, and that CTmin readouts of decontaminated sputum samples should be interpreted with caution.
Rachow , A , Saathoff , E , Mtafya , B , Mapamba , D , Mangu , C , Rojas-Ponce , G , Ntinginya , N E , Boeree , M , Heinrich , N , Gillespie , S H , Hoelscher , M & PanACEA Consortium 2018 , ' The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay ' , Tuberculosis , vol. In press . https://doi.org/10.1016/j.tube.2018.04.001
© 2018 Elsevier Ltd. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1016/j.tube.2018.04.001