The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay
Abstract
The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs. Our data indicate that NALC/NaOH-decontamination has an effect on the performance of the Xpert assay, and that CTmin readouts of decontaminated sputum samples should be interpreted with caution.
Citation
Rachow , A , Saathoff , E , Mtafya , B , Mapamba , D , Mangu , C , Rojas-Ponce , G , Ntinginya , N E , Boeree , M , Heinrich , N , Gillespie , S H , Hoelscher , M & PanACEA Consortium 2018 , ' The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay ' , Tuberculosis , vol. In press . https://doi.org/10.1016/j.tube.2018.04.001
Publication
Tuberculosis
Status
Peer reviewed
ISSN
1472-9792Type
Journal article
Description
The study was supported by the German Ministry of Education and Research (BMBF, Grant No. 01KA0901 ) and the European and Developing Countries Clinical Trials Partnership (EDCTP, Project-ID: IP.2007.32011.011 ).Collections
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