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Light sheet microscopy with acoustic sample confinement
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dc.contributor.author | Yang, Zhengyi | |
dc.contributor.author | Cole, Katy L.H. | |
dc.contributor.author | Qiu, Yongqiang | |
dc.contributor.author | Somorjai, Ildiko Maureen Lara | |
dc.contributor.author | Wijesinghe, Philip | |
dc.contributor.author | Nylk, Jonathan | |
dc.contributor.author | Cochran, Sandy | |
dc.contributor.author | Spalding, Gabriel C. | |
dc.contributor.author | Lyons, David A. | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2019-02-08T16:30:05Z | |
dc.date.available | 2019-02-08T16:30:05Z | |
dc.date.issued | 2019-02-08 | |
dc.identifier | 250513601 | |
dc.identifier | db1e0b53-4b8a-4f63-97cc-51dc255e7e75 | |
dc.identifier | 85061240428 | |
dc.identifier | 000458175300018 | |
dc.identifier.citation | Yang , Z , Cole , K L H , Qiu , Y , Somorjai , I M L , Wijesinghe , P , Nylk , J , Cochran , S , Spalding , G C , Lyons , D A & Dholakia , K 2019 , ' Light sheet microscopy with acoustic sample confinement ' , Nature Communications , vol. 10 , 669 . https://doi.org/10.1038/s41467-019-08514-5 | en |
dc.identifier.issn | 2041-1723 | |
dc.identifier.other | ORCID: /0000-0002-2977-4929/work/53857092 | |
dc.identifier.other | ORCID: /0000-0001-5243-6664/work/53857099 | |
dc.identifier.other | ORCID: /0000-0002-8378-7261/work/54516680 | |
dc.identifier.uri | https://hdl.handle.net/10023/17025 | |
dc.description | Funding: UK Engineering and Physical Sciences Research Council (EPSRC) (grant numbers EP/P030017/1, EP/M000869/1 and EP/R004854/1) for funding. Work in the Somorjai lab is supported by the Wellcome Trust ISSF, the RS Macdonald Trust Charitable and EU Horizon 2020 INFRADEV ”CORBEL” (grant number 654248). | en |
dc.description.abstract | Contactless sample confinement would enable a whole host of new studies in developmental biology and neuroscience, in particular, when combined with long-term, wide-field optical imaging. To achieve this goal, we demonstrate a contactless acoustic gradient force trap for sample confinement in light sheet microscopy. Our approach allows the integration of real-time environmentally controlled experiments with wide-field low photo-toxic imaging, which we demonstrate on a variety of marine animal embryos and larvae. To illustrate the key advantages of our approach, we provide quantitative data for the dynamic response of the heartbeat of zebrafish larvae to verapamil and norepinephrine, which are known to affect cardiovascular function. Optical flow analysis allows us to explore the cardiac cycle of the zebrafish and determine the changes in contractile volume within the heart. Overcoming the restrictions of sample immobilisation and mounting can open up a broad range of studies, with real-time drug-based assays and biomechanical analyses. | |
dc.format.extent | 8 | |
dc.format.extent | 3076182 | |
dc.language.iso | eng | |
dc.relation.ispartof | Nature Communications | en |
dc.subject | QC Physics | en |
dc.subject | T Technology | en |
dc.subject | DAS | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | T | en |
dc.title | Light sheet microscopy with acoustic sample confinement | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.contributor.sponsor | European Commission | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Marine Alliance for Science & Technology Scotland | en |
dc.contributor.institution | University of St Andrews. Scottish Oceans Institute | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. Centre for Biophotonics | en |
dc.identifier.doi | 10.1038/s41467-019-08514-5 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/P030017/1 | en |
dc.identifier.grantnumber | EP/M000869/1 | en |
dc.identifier.grantnumber | EP/R004854/1 | en |
dc.identifier.grantnumber | en | |
dc.identifier.grantnumber | 654248 | en |
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