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Light sheet microscopy with acoustic sample confinement

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Yang_2019_Light_sheet_microscopy_NatComms_669_CC.pdf (2.933Mb)
Date
08/02/2019
Author
Yang, Zhengyi
Cole, Katy L.H.
Qiu, Yongqiang
Somorjai, Ildiko Maureen Lara
Wijesinghe, Philip
Nylk, Jonathan
Cochran, Sandy
Spalding, Gabriel C.
Lyons, David A.
Dholakia, Kishan
Keywords
QC Physics
T Technology
DAS
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Abstract
Contactless sample confinement would enable a whole host of new studies in developmental biology and neuroscience, in particular, when combined with long-term, wide-field optical imaging. To achieve this goal, we demonstrate a contactless acoustic gradient force trap for sample confinement in light sheet microscopy. Our approach allows the integration of real-time environmentally controlled experiments with wide-field low photo-toxic imaging, which we demonstrate on a variety of marine animal embryos and larvae. To illustrate the key advantages of our approach, we provide quantitative data for the dynamic response of the heartbeat of zebrafish larvae to verapamil and norepinephrine, which are known to affect cardiovascular function. Optical flow analysis allows us to explore the cardiac cycle of the zebrafish and determine the changes in contractile volume within the heart. Overcoming the restrictions of sample immobilisation and mounting can open up a broad range of studies, with real-time drug-based assays and biomechanical analyses.
Citation
Yang , Z , Cole , K L H , Qiu , Y , Somorjai , I M L , Wijesinghe , P , Nylk , J , Cochran , S , Spalding , G C , Lyons , D A & Dholakia , K 2019 , ' Light sheet microscopy with acoustic sample confinement ' , Nature Communications , vol. 10 , 669 . https://doi.org/10.1038/s41467-019-08514-5
Publication
Nature Communications
Status
Peer reviewed
DOI
https://doi.org/10.1038/s41467-019-08514-5
ISSN
2041-1723
Type
Journal article
Rights
© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Description
Funding: UK Engineering and Physical Sciences Research Council (EPSRC) (grant numbers EP/P030017/1, EP/M000869/1 and EP/R004854/1) for funding. Work in the Somorjai lab is supported by the Wellcome Trust ISSF, the RS Macdonald Trust Charitable and EU Horizon 2020 INFRADEV ”CORBEL” (grant number 654248).
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/17025

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