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Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

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dc.contributor.authorBaron, Vincent O
dc.contributor.authorChen, Mingzhou
dc.contributor.authorClark, Simon O
dc.contributor.authorWilliams, Ann
dc.contributor.authorDholakia, Kishan
dc.contributor.authorGillespie, Stephen H
dc.contributor.editorGillespie, Stephen
dc.date.accessioned2019-01-11T00:30:50Z
dc.date.available2019-01-11T00:30:50Z
dc.date.issued2018
dc.identifier252482462
dc.identifiera6f41507-3e34-42b7-ab8a-d49704b22838
dc.identifier29322457
dc.identifier85040655458
dc.identifier000431336200005
dc.identifier.citationBaron , V O , Chen , M , Clark , S O , Williams , A , Dholakia , K & Gillespie , S H 2018 , Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy . in S Gillespie (ed.) , Antibiotic Resistance Protocols . Methods in Molecular Biology , vol. 1736 , Humana Press/Springer , New York, NY , pp. 41-50 . https://doi.org/10.1007/978-1-4939-7638-6_4en
dc.identifier.isbn9781493976362
dc.identifier.isbn9781493976386
dc.identifier.issn1064-3745
dc.identifier.otherORCID: /0000-0001-6537-7712/work/42023881
dc.identifier.otherORCID: /0000-0002-6190-5167/work/47136397
dc.identifier.urihttps://hdl.handle.net/10023/16841
dc.description.abstractRaman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.
dc.format.extent10
dc.format.extent577703
dc.language.isoeng
dc.publisherHumana Press/Springer
dc.relation.ispartofAntibiotic Resistance Protocolsen
dc.relation.ispartofseriesMethods in Molecular Biologyen
dc.subjectRaman spectroscopyen
dc.subjectMycobacteriaen
dc.subjectPhenotypic resistanceen
dc.subjectLipidsen
dc.subjectQH301 Biologyen
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subject.lccQH301en
dc.subject.lccRMen
dc.titleDetecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopyen
dc.typeBook itemen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. Global Health Implementation Groupen
dc.contributor.institutionUniversity of St Andrews. Gillespie Groupen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.identifier.doi10.1007/978-1-4939-7638-6_4
dc.date.embargoedUntil2019-01-11


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