Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy
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Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.
Baron , V O , Chen , M , Clark , S O , Williams , A , Dholakia , K & Gillespie , S H 2018 , Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy . in S Gillespie (ed.) , Antibiotic Resistance Protocols . Methods in Molecular Biology , vol. 1736 , Humana Press/Springer , New York, NY , pp. 41-50 . DOI: 10.1007/978-1-4939-7638-6_4
Antibiotic Resistance Protocols
© 2018, Springer Science+Business Media, LLC. This work has been made available online by kind permission of the publisher. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1007/978-1-4939-7638-6_4
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