Files in this item
Pulse EPR distance measurements to study multimers and multimerisation
Item metadata
dc.contributor.author | Ackermann, Katrin | |
dc.contributor.author | Bode, Bela E. | |
dc.date.accessioned | 2019-01-05T00:33:31Z | |
dc.date.available | 2019-01-05T00:33:31Z | |
dc.date.issued | 2018 | |
dc.identifier | 251804307 | |
dc.identifier | 7b536fb2-c4a3-4512-817b-7fbddbea90d2 | |
dc.identifier | 85040973180 | |
dc.identifier | 000431589200001 | |
dc.identifier.citation | Ackermann , K & Bode , B E 2018 , ' Pulse EPR distance measurements to study multimers and multimerisation ' , Molecular Physics , vol. 116 , no. 12 , pp. 1513-1521 . https://doi.org/10.1080/00268976.2017.1421324 | en |
dc.identifier.issn | 0026-8976 | |
dc.identifier.other | ORCID: /0000-0002-3384-271X/work/40535193 | |
dc.identifier.uri | https://hdl.handle.net/10023/16782 | |
dc.description | This work was supported by funding from the European Union (Marie Curie Actions REA 334496), the Carnegie Trust (70098), the EPSRC (EP/M024660/1) and a Wellcome Trust multi-user equipment grant (099149/Z/12/Z). | en |
dc.description.abstract | Pulse dipolar electron paramagnetic resonance (PD-EPR) has become a powerful tool for structural biology determining distances on the nanometre scale. Recent advances in hardware, methodology, and data analysis have widened the scope to complex biological systems. PD-EPR can be applied to systems containing lowly populated conformers or displaying large intrinsic flexibility, making them all but intractable for cryo-electron microscopy and crystallography. Membrane protein applications are of particular interest due to the intrinsic difficulties for obtaining high-resolution structures of all relevant conformations. Many drug targets involved in critical cell functions are multimeric channels or transporters. Here, common approaches for introducing spin labels for PD-EPR cause the presence of more than two electron spins per multimeric complex. This requires careful experimental design to overcome detrimental multi-spin effects and to secure sufficient distance resolution in presence of multiple distances. In addition to obtaining mere distances, PD-EPR can also provide information on multimerisation degrees allowing to study binding equilibria and to determine dissociation constants. | |
dc.format.extent | 9 | |
dc.format.extent | 746579 | |
dc.language.iso | eng | |
dc.relation.ispartof | Molecular Physics | en |
dc.subject | Electron paramagnetic resonance | en |
dc.subject | PELDOR | en |
dc.subject | DEER | en |
dc.subject | RIDME | en |
dc.subject | Multi-spin | en |
dc.subject | QD Chemistry | en |
dc.subject | QC Physics | en |
dc.subject | DAS | en |
dc.subject.lcc | QD | en |
dc.subject.lcc | QC | en |
dc.title | Pulse EPR distance measurements to study multimers and multimerisation | en |
dc.type | Journal article | en |
dc.contributor.sponsor | Carnegie Trust | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.contributor.sponsor | European Commission | en |
dc.contributor.institution | University of St Andrews. School of Chemistry | en |
dc.contributor.institution | University of St Andrews. Centre of Magnetic Resonance | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. EaSTCHEM | en |
dc.identifier.doi | 10.1080/00268976.2017.1421324 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2019-01-05 | |
dc.identifier.grantnumber | 70098 | en |
dc.identifier.grantnumber | EP/M024660/1 | en |
dc.identifier.grantnumber | 099149/Z/12/Z | en |
dc.identifier.grantnumber | en |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.