Sharpening emitter localization in front of a tuned mirror
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Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tunedmirror2–4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor oftwo. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.
Heil , H S , Schreiber , B , Götz , R , Emmerling , M , Dabauvalle , M-C , Krohne , G , Höfling , S , Kamp , M , Sauer , M & Heinze , K G 2018 , ' Sharpening emitter localization in front of a tuned mirror ' , Light: Science & Applications , vol. 7 , 99 . https://doi.org/10.1038/s41377-018-0104-z
Light: Science & Applications
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