Sharpening emitter localization in front of a tuned mirror
Abstract
Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tunedmirror2–4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor oftwo. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.
Citation
Heil , H S , Schreiber , B , Götz , R , Emmerling , M , Dabauvalle , M-C , Krohne , G , Höfling , S , Kamp , M , Sauer , M & Heinze , K G 2018 , ' Sharpening emitter localization in front of a tuned mirror ' , Light: Science & Applications , vol. 7 , 99 . https://doi.org/10.1038/s41377-018-0104-z
Publication
Light: Science & Applications
Status
Peer reviewed
ISSN
2047-7538Type
Journal article
Rights
© The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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