Advances in the synthesis of nitroxide radicals for use in biomolecule spin labelling
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EPR spectroscopy is an increasingly useful analytical tool to probe biomolecule structure, dynamic behaviour, and interactions. Nitroxide radicals are the most commonly used radical probe in EPR experiments, and many methods have been developed for their synthesis, as well as incorporation into biomolecules using site-directed spin labelling. In this Tutorial Review, we discuss the most practical methods for the synthesis of nitroxides, focusing on the tunability of their structures, the manipulation of their sidechains into spin labelling handles, and their installation into biomolecules.
Haugland , M , Lovett , J E & Anderson , E 2018 , ' Advances in the synthesis of nitroxide radicals for use in biomolecule spin labelling ' Chemical Society Reviews , vol. 47 , no. 3 , pp. 668-680 . https://doi.org/10.1039/C6CS00550K
Chemical Society Reviews
© 2017, the Author(s). This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1039/C6CS00550K
DescriptionFunding: UK EPSRC (EP/L022044/1), and the Royal Society for a University Research Fellowship (JEL).
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