St Andrews Research Repository

St Andrews University Home
View Item 
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Mumps virus Enders strain is sensitive to interferon (IFN) despite encoding a functional IFN antagonist

Thumbnail
View/Open
RandallGenVir90-MumpsVirus.pdf (378.5Kb)
Date
11/2009
Author
Young, D F
Galiano, M
Lemon, K
Chen, Y-H
Andrejeva, Jelena
Duprex, P
Rima, B K
Randall, Richard Edward
Keywords
Dependent rna-polymerase
V-protein
Antiviral responses
RIG-I
Parainfluenza virus-5
Gene-expression
Degredation
Induction
Cells
Transduction
QR355 Virology
Metadata
Show full item record
Altmetrics Handle Statistics
Altmetrics DOI Statistics
Abstract
Although the Enders strain of mumps virus (MuV) encodes a functional V protein that acts as an interferon (IFN) antagonist, in multi-cycle growth assays MuV Enders grew poorly in naive ('IFN-competent' Hep2) cells but grew to high titres in 'IFN-compromised' Hep2 cells. Even so, the growth rate of MuV Enders was significantly slower in 'IFN-compromised' Hep2 cells when compared with its replication rate in Vero cells and with the replication rate of parainfluenza virus type 5 (a closely related paramyxovirus) in both naive and 'IFN-compromised' Hep2 cells. This suggests that a consequence of slower growth is that the IFN system of naive Hep2 cells can respond quickly enough to control the growth of MuV Enders. This is supported by the finding that rapidly growing variants of MuV Enders that were selected on 'IFN-compromised' Hep2 cells (i.e. in the absence of any selection pressure exerted by the IFN response) also grew to high titres on naive Hep2 cells. Sequencing of the complete genome of one of these variants identified a single point mutation that resulted in a substitution of a conserved asparagine by histidine at position 498 of the haemagglutinin-neuraminidase protein, although this mutation was not present in all rapidly growing variants. These results support the concept that there is a race between the ability of a cell to detect and respond to virus infection and the ability of a virus to block the IFN response. Importantly, this emphasizes that factors other than viral IFN antagonists influence the sensitivity of viruses to IFN.
Citation
Young , D F , Galiano , M , Lemon , K , Chen , Y-H , Andrejeva , J , Duprex , P , Rima , B K & Randall , R E 2009 , ' Mumps virus Enders strain is sensitive to interferon (IFN) despite encoding a functional IFN antagonist ' , Journal of General Virology , vol. 90 , no. 11 , pp. 2731-2738 . https://doi.org/10.1099/vir.0.013722-0
Publication
Journal of General Virology
Status
Peer reviewed
DOI
https://doi.org/10.1099/vir.0.013722-0
ISSN
0022-1317
Type
Journal article
Rights
(c)2009 SGM. Article available under publisher's Open Option
Collections
  • University of St Andrews Research
URL
http://www.scopus.com/inward/record.url?scp=70350443463&partnerID=8YFLogxK
URI
http://hdl.handle.net/10023/1640

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Advanced Search

Browse

All of RepositoryCommunities & CollectionsBy Issue DateNamesTitlesSubjectsClassificationTypeFunderThis CollectionBy Issue DateNamesTitlesSubjectsClassificationTypeFunder

My Account

Login

Open Access

To find out how you can benefit from open access to research, see our library web pages and Open Access blog. For open access help contact: openaccess@st-andrews.ac.uk.

Accessibility

Read our Accessibility statement.

How to submit research papers

The full text of research papers can be submitted to the repository via Pure, the University's research information system. For help see our guide: How to deposit in Pure.

Electronic thesis deposit

Help with deposit.

Repository help

For repository help contact: Digital-Repository@st-andrews.ac.uk.

Give Feedback

Cookie policy

This site may use cookies. Please see Terms and Conditions.

Usage statistics

COUNTER-compliant statistics on downloads from the repository are available from the IRUS-UK Service. Contact us for information.

© University of St Andrews Library

University of St Andrews is a charity registered in Scotland, No SC013532.

  • Facebook
  • Twitter