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dc.contributor.authorEscobet-Montalbán, Adrià
dc.contributor.authorSpesyvtsev, Roman
dc.contributor.authorChen, Mingzhou
dc.contributor.authorSaber, Wardiya Afshar
dc.contributor.authorAndrews, Melissa
dc.contributor.authorHerrington, C. Simon
dc.contributor.authorMazilu, Michael
dc.contributor.authorDholakia, Kishan
dc.identifier.citationEscobet-Montalbán , A , Spesyvtsev , R , Chen , M , Saber , W A , Andrews , M , Herrington , C S , Mazilu , M & Dholakia , K 2018 , ' Wide-field multiphoton imaging through scattering media without correction ' , Science Advances , vol. 4 , no. 10 , eaau1338 , pp. 1-9 .
dc.identifier.otherPURE: 252078679
dc.identifier.otherPURE UUID: 52b172cc-2b1b-4921-9350-591f6863002d
dc.identifier.otherScopus: 85054904640
dc.identifier.otherORCID: /0000-0002-6190-5167/work/49308257
dc.identifier.otherWOS: 000449221200055
dc.descriptionFunding: This work is supported by the UK Engineering and Physical Sciences Research Council for funding through grants EP/P030017/1 and EP/M000869/1, and has received funding from the European Union’s Horizon 2020 Programme through the project Advanced BiomEdical OPTICAL Imaging and Data Analysis (BE-OPTICAL) under grant agreement no. 675512, The Cunningham Trust and The RS MacDonald Charitable Trust. KD acknowledges the financial support of Elizabeth Killick and Susan Gurney.en
dc.description.abstractOptical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the biomedical sciences. While the advances have been startling, the key unmet challenge to date in all forms of optical imaging is to penetrate deeper. A number of schemes implement aberration correction or the use of complex photonics to address this need. In contrast, we approach this challenge by implementing a scheme that requires no a priori information about the medium nor its properties. Exploiting temporal focusing and single-pixel detection in our innovative scheme, we obtain wide-field two-photon images through various turbid media including a scattering phantom and tissue reaching a depth of up to seven scattering mean free path lengths. Our results show that it competes favorably with standard point-scanning two-photon imaging, with up to a fivefold improvement in signal-to-background ratio while showing significantly lower photobleaching.
dc.relation.ispartofScience Advancesen
dc.rightsCopyright © 2018 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectQC Physicsen
dc.titleWide-field multiphoton imaging through scattering media without correctionen
dc.typeJournal articleen
dc.contributor.sponsorEuropean Commissionen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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