Wide-field multiphoton imaging through scattering media without correction
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Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the biomedical sciences. While the advances have been startling, the key unmet challenge to date in all forms of optical imaging is to penetrate deeper. A number of schemes implement aberration correction or the use of complex photonics to address this need. In contrast, we approach this challenge by implementing a scheme that requires no a priori information about the medium nor its properties. Exploiting temporal focusing and single-pixel detection in our innovative scheme, we obtain wide-field two-photon images through various turbid media including a scattering phantom and tissue reaching a depth of up to seven scattering mean free path lengths. Our results show that it competes favorably with standard point-scanning two-photon imaging, with up to a fivefold improvement in signal-to-background ratio while showing significantly lower photobleaching.
Escobet-Montalbán , A , Spesyvtsev , R , Chen , M , Saber , W A , Andrews , M , Herrington , C S , Mazilu , M & Dholakia , K 2018 , ' Wide-field multiphoton imaging through scattering media without correction ' , Science Advances , vol. 4 , no. 10 , eaau1338 , pp. 1-9 . https://doi.org/10.1126/sciadv.aau1338
Copyright © 2018 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DescriptionFunding: This work is supported by the UK Engineering and Physical Sciences Research Council for funding through grants EP/P030017/1 and EP/M000869/1, and has received funding from the European Union’s Horizon 2020 Programme through the project Advanced BiomEdical OPTICAL Imaging and Data Analysis (BE-OPTICAL) under grant agreement no. 675512, The Cunningham Trust and The RS MacDonald Charitable Trust. KD acknowledges the financial support of Elizabeth Killick and Susan Gurney.
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