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dc.contributor.authorAhmed, Mohamed I. M.
dc.contributor.authorNtinginya, Nyanda E.
dc.contributor.authorKibiki, Gibson
dc.contributor.authorMtafya, Bariki A
dc.contributor.authorSemvua, Hadija
dc.contributor.authorMpagama, Stellah
dc.contributor.authorMtabho, Charles
dc.contributor.authorSaathoff, Elmar
dc.contributor.authorHeld, Kathrin
dc.contributor.authorLoose, Rebecca
dc.contributor.authorKroidl, Inge
dc.contributor.authorChachage, Mkunde
dc.contributor.authorBoth, Ulrich von
dc.contributor.authorHaule, Antelmo
dc.contributor.authorMekota, Anna-Maria
dc.contributor.authorBoeree, Martin J.
dc.contributor.authorGillespie, Stephen H.
dc.contributor.authorHoelscher, Michael
dc.contributor.authorHeinrich, Norbert
dc.contributor.authorGeldmacher, Christof
dc.contributor.authorPan African Consortium for the Evaluation of Antituberculosis Antibiotics (PanACEA)
dc.date.accessioned2018-10-10T16:30:06Z
dc.date.available2018-10-10T16:30:06Z
dc.date.issued2018-09-28
dc.identifier.citationAhmed , M I M , Ntinginya , N E , Kibiki , G , Mtafya , B A , Semvua , H , Mpagama , S , Mtabho , C , Saathoff , E , Held , K , Loose , R , Kroidl , I , Chachage , M , Both , U V , Haule , A , Mekota , A-M , Boeree , M J , Gillespie , S H , Hoelscher , M , Heinrich , N , Geldmacher , C & Pan African Consortium for the Evaluation of Antituberculosis Antibiotics (PanACEA) 2018 , ' Phenotypic changes on Mycobacterium tuberculosis -specific CD4 T cells as surrogate markers for tuberculosis treatment efficacy ' , Frontiers in Immunology , vol. 9 , 2247 . https://doi.org/10.3389/fimmu.2018.02247en
dc.identifier.issn1664-3224
dc.identifier.otherPURE: 256162851
dc.identifier.otherPURE UUID: 95cf5b93-d98a-447f-a973-b03c65b6af84
dc.identifier.otherRIS: urn:2F5862A65E2AFF22656A22605DE4DF3C
dc.identifier.otherORCID: /0000-0001-6537-7712/work/54819103
dc.identifier.otherWOS: 000445920900001
dc.identifier.otherScopus: 85054892786
dc.identifier.urihttps://hdl.handle.net/10023/16180
dc.descriptionThis work was supported by the European & Developing Countries Clinical Trials Partnership (EDCTP) (PanACEA, grant numbers IP.2007.32011.011, IP.2007.32011.012, IP.2007.32011.013), by the German Ministry for Education and Research (BMBF; Grant No. 01KA0901) by the European Commission, DG XII, INCO-DC (grant ICA-CT-2002-10048) and by the German Center for Infection Research (DZIF).en
dc.description.abstractBackground: The analysis of phenotypic characteristics on Mycobacterium tuberculosis (MTB)-specific T cells is a promising approach for the diagnosis of active tuberculosis (aTB) and for monitoring treatment success. We therefore studied phenotypic changes on MTB-specific CD4 T cells upon anti-tuberculosis treatment initiation in relation to the treatment response as determined by sputum culture. Methods: Peripheral blood mononuclear cells from subjects with latent MTB infection (n=16) and aTB (n=39) at baseline, week 9, 12 and 26 (end of treatment) were analyzed after intracellular interferon gamma staining and overnight stimulation with tuberculin. Liquid sputum cultures were performed weekly until week 12 and during 4 visits until week 26. Results: T cell activation marker expression on MTB-specific CD4 T cells differed significantly between subjects with aTB and latent MTB infection with no overlap for the frequencies of CD38pos and Ki67pos cells (both p < 0.0001). At 9 weeks after anti-TB treatment initiation the frequencies of activation marker (CD38, HLA-DR, Ki67) positive MTB-specific, but not total CD4 T cells, were significantly reduced (p < 0.0001). Treatment induced phenotypic changes from baseline until week 9 and until week 12 differed substantially between individual aTB patients and correlated with an individual's time to stable sputum culture conversion for expression of CD38 and HLA-DR (both p < 0.05). In contrast, the frequencies of maturation marker CD27 positive MTB-specific CD4 T cells remained largely unchanged until week 26 and significantly differed between subjects with treated TB disease and latent MTB infection (p = 0.0003). Discussion: Phenotypic changes of MTB-specific T cells are potential surrogate markers for tuberculosis treatment efficacy and can help to discriminate between aTB (profile: CD38pos, CD27low), treated TB (CD38neg, CD27low), and latent MTB infection (CD38neg, CD27high).
dc.format.extent13
dc.language.isoeng
dc.relation.ispartofFrontiers in Immunologyen
dc.rightsCopyright © 2018 Ahmed, Ntinginya, Kibiki, Mtafya, Semvua, Mpagama, Mtabho, Saathoff, Held, Loose, Kroidl, Chachage, von Both, Haule, Mekota, Boeree, Gillespie, Hoelscher, Heinrich and Geldmacher. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.subjectTAM-TB assayen
dc.subjectTuberculosisen
dc.subjectTreatment monitoringen
dc.subjectMycobacterium tuberculosis-specific T cellsen
dc.subjectSerial sputum cultureen
dc.subjectBiomarkeren
dc.subjectQR180 Immunologyen
dc.subjectDASen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subject.lccQR180en
dc.titlePhenotypic changes on Mycobacterium tuberculosis-specific CD4 T cells as surrogate markers for tuberculosis treatment efficacyen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. Global Health Implementation Groupen
dc.contributor.institutionUniversity of St Andrews. Gillespie Groupen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.identifier.doihttps://doi.org/10.3389/fimmu.2018.02247
dc.description.statusPeer revieweden


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