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Phenotypic changes on Mycobacterium tuberculosis-specific CD4 T cells as surrogate markers for tuberculosis treatment efficacy

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Date
28/09/2018
Author
Ahmed, Mohamed I. M.
Ntinginya, Nyanda E.
Kibiki, Gibson
Mtafya, Bariki A
Semvua, Hadija
Mpagama, Stellah
Mtabho, Charles
Saathoff, Elmar
Held, Kathrin
Loose, Rebecca
Kroidl, Inge
Chachage, Mkunde
Both, Ulrich von
Haule, Antelmo
Mekota, Anna-Maria
Boeree, Martin J.
Gillespie, Stephen H.
Hoelscher, Michael
Heinrich, Norbert
Geldmacher, Christof
Pan African Consortium for the Evaluation of Antituberculosis Antibiotics (PanACEA)
Keywords
TAM-TB assay
Tuberculosis
Treatment monitoring
Mycobacterium tuberculosis-specific T cells
Serial sputum culture
Biomarker
QR180 Immunology
DAS
Metadata
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Abstract
Background: The analysis of phenotypic characteristics on Mycobacterium tuberculosis (MTB)-specific T cells is a promising approach for the diagnosis of active tuberculosis (aTB) and for monitoring treatment success. We therefore studied phenotypic changes on MTB-specific CD4 T cells upon anti-tuberculosis treatment initiation in relation to the treatment response as determined by sputum culture. Methods: Peripheral blood mononuclear cells from subjects with latent MTB infection (n=16) and aTB (n=39) at baseline, week 9, 12 and 26 (end of treatment) were analyzed after intracellular interferon gamma staining and overnight stimulation with tuberculin. Liquid sputum cultures were performed weekly until week 12 and during 4 visits until week 26. Results: T cell activation marker expression on MTB-specific CD4 T cells differed significantly between subjects with aTB and latent MTB infection with no overlap for the frequencies of CD38pos and Ki67pos cells (both p < 0.0001). At 9 weeks after anti-TB treatment initiation the frequencies of activation marker (CD38, HLA-DR, Ki67) positive MTB-specific, but not total CD4 T cells, were significantly reduced (p < 0.0001). Treatment induced phenotypic changes from baseline until week 9 and until week 12 differed substantially between individual aTB patients and correlated with an individual's time to stable sputum culture conversion for expression of CD38 and HLA-DR (both p < 0.05). In contrast, the frequencies of maturation marker CD27 positive MTB-specific CD4 T cells remained largely unchanged until week 26 and significantly differed between subjects with treated TB disease and latent MTB infection (p = 0.0003). Discussion: Phenotypic changes of MTB-specific T cells are potential surrogate markers for tuberculosis treatment efficacy and can help to discriminate between aTB (profile: CD38pos, CD27low), treated TB (CD38neg, CD27low), and latent MTB infection (CD38neg, CD27high).
Citation
Ahmed , M I M , Ntinginya , N E , Kibiki , G , Mtafya , B A , Semvua , H , Mpagama , S , Mtabho , C , Saathoff , E , Held , K , Loose , R , Kroidl , I , Chachage , M , Both , U V , Haule , A , Mekota , A-M , Boeree , M J , Gillespie , S H , Hoelscher , M , Heinrich , N , Geldmacher , C & Pan African Consortium for the Evaluation of Antituberculosis Antibiotics (PanACEA) 2018 , ' Phenotypic changes on Mycobacterium tuberculosis -specific CD4 T cells as surrogate markers for tuberculosis treatment efficacy ' , Frontiers in Immunology , vol. 9 , 2247 . https://doi.org/10.3389/fimmu.2018.02247
Publication
Frontiers in Immunology
Status
Peer reviewed
DOI
https://doi.org/10.3389/fimmu.2018.02247
ISSN
1664-3224
Type
Journal article
Rights
Copyright © 2018 Ahmed, Ntinginya, Kibiki, Mtafya, Semvua, Mpagama, Mtabho, Saathoff, Held, Loose, Kroidl, Chachage, von Both, Haule, Mekota, Boeree, Gillespie, Hoelscher, Heinrich and Geldmacher. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Description
This work was supported by the European & Developing Countries Clinical Trials Partnership (EDCTP) (PanACEA, grant numbers IP.2007.32011.011, IP.2007.32011.012, IP.2007.32011.013), by the German Ministry for Education and Research (BMBF; Grant No. 01KA0901) by the European Commission, DG XII, INCO-DC (grant ICA-CT-2002-10048) and by the German Center for Infection Research (DZIF).
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/16180

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