Depth-resolved multimodal imaging : wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography
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A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth co-localization obtained from the OCT, we can penetrate 1.2mm deep into the strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (Polystyrene) with a spatial offset. Our approach is also capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter within a brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.
Chen , M , Mas , J , Forbes , L H , Andrews , M R & Dholakia , K 2018 , ' Depth-resolved multimodal imaging : wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography ' , Journal of Biophotonics , vol. 11 , no. 1 , e201700129 . https://doi.org/10.1002/jbio.201700129
Journal of Biophotonics
© 2017, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at onlinelibrary.wiley.com / https://doi.org/10.1002/jbio.201700129
DescriptionFunding: UK Engineering and Physical Sciences Research Council (EPSRC: EP/J01771X/1, EP/M000869/1), the European Union FAMOS project (FP7 ICT, 317744) and the RS MacDonald Charitable Trust for funding.
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