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Diversity of Streptococcus equi subsp. zooepidemicus strains isolated from the Spanish sheep and goat population and the identification, function and prevalence of a novel arbutin utilisation system

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Date
08/2017
Author
Steward, Karen F.
Robinson, Carl
Holden, Matthew T. G.
Harris, Simon R.
Ros, Ana Fernández
Pérez, Gema Chacón
Baselga, Rafael
Waller, Andrew S.
Keywords
Streptococcus zooepedemicus
Mastitis
Goat
Sheep
Arbutin utilisation
PTS system acquisition
QR Microbiology
SF Animal culture
NDAS
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Abstract
The zoonotic bacterium Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is a diverse, opportunistic pathogen that can cause mastitis in dairy sheep and goats. We used multilocus sequence typing (MLST) to define the genetic diversity of 60 isolates of S. zooepidemicus, which were recovered from sheep and goats in Spain between 2003 and 2010. We identify a novel clonal complex based on sequence type (ST), ST-236, which accounted for 39 of the 60 isolates. A representative ST-236 strain, S. zooepidemicus strain C7 (SzC7), was sequenced and interrogated for the presence of novel nutritional uptake or utilisation systems, the acquisition of which have previously been shown to be important for environmental adaptation in other streptococcal pathogens. A novel phosphoenolpyruvate sugar phosphotransferase system (PTS), which enabled the utilisation of arbutin, was identified. Functionality of the PTS was confirmed following deletion of the PTS from SzC7. Arbutin is found in multiple animal foodstuffs and we propose that the ability to utilise arbutin may have conferred a selective advantage to strains infecting animals, the diet of which contains this sugar.
Citation
Steward , K F , Robinson , C , Holden , M T G , Harris , S R , Ros , A F , Pérez , G C , Baselga , R & Waller , A S 2017 , ' Diversity of Streptococcus equi subsp. zooepidemicus strains isolated from the Spanish sheep and goat population and the identification, function and prevalence of a novel arbutin utilisation system ' , Veterinary Microbiology , vol. 207 , pp. 231-238 . https://doi.org/10.1016/j.vetmic.2017.06.020
Publication
Veterinary Microbiology
Status
Peer reviewed
DOI
https://doi.org/10.1016/j.vetmic.2017.06.020
ISSN
0378-1135
Type
Journal article
Rights
© 2017, Elsevier BV. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at www.sciencedirect.com / http://dx.doi.org/10.1016/j.vetmic.2017.06.020
Description
This work was supported by the Animal Health Trust.
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  • University of St Andrews Research
URL
http://www.sciencedirect.com/science/article/pii/S0378113517305850#appd002
URI
http://hdl.handle.net/10023/14765

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