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dc.contributor.authorHaslehurst, Peter
dc.contributor.authorYang, Zhengyi
dc.contributor.authorDholakia, Kishan
dc.contributor.authorEmptage, Nigel
dc.identifier.citationHaslehurst , P , Yang , Z , Dholakia , K & Emptage , N 2018 , ' Fast volume-scanning light sheet microscopy reveals transient neuronal events ' , Biomedical Optics Express , vol. 9 , no. 5 , pp. 2154-2167 .
dc.identifier.otherPURE: 252440426
dc.identifier.otherPURE UUID: f8640611-61b7-40cd-a711-00b17e853189
dc.identifier.otherScopus: 85046374226
dc.identifier.otherWOS: 000431181700012
dc.descriptionFunding: UK EPSRC EP/P030017/1.en
dc.description.abstractLight sheet fluorescence microscopy offers considerable potential to the cellular neuroscience community as it makes it possible to image extensive areas of neuronal structures, such as axons or dendrites, with a low light budget, thereby minimizing phototoxicity. However, the shallow depth of a light sheet, which is critical for achieving high contrast, well resolved images, adds a significant challenge if fast functional imaging is also required, as multiple images need to be collected across several image planes. Consequently, fast functional imaging of neurons is typically restricted to a small tissue volume where part of the neuronal structure lies within the plane of a single image. Here we describe a method by which fast functional imaging can be achieved across a much larger tissue volume; a custom-built light sheet microscope is presented that includes a synchronized galvo mirror and electrically tunable lens, enabling high speed acquisition of images across a configurable depth. We assess the utility of this technique by acquiring fast functional Ca2+ imaging data across a neuron’s dendritic arbour in mammalian brain tissue.
dc.relation.ispartofBiomedical Optics Expressen
dc.rightsCopyright 2017 the Authors. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.en
dc.subjectQC Physicsen
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectT Technologyen
dc.titleFast volume-scanning light sheet microscopy reveals transient neuronal eventsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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