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Fast volume-scanning light sheet microscopy reveals transient neuronal events

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Haslehurst_2018_Fast_volume_BOE_2154_CC.pdf (5.730Mb)
Date
01/05/2018
Author
Haslehurst, Peter
Yang, Zhengyi
Dholakia, Kishan
Emptage, Nigel
Keywords
QC Physics
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
RM Therapeutics. Pharmacology
T Technology
NDAS
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Abstract
Light sheet fluorescence microscopy offers considerable potential to the cellular neuroscience community as it makes it possible to image extensive areas of neuronal structures, such as axons or dendrites, with a low light budget, thereby minimizing phototoxicity. However, the shallow depth of a light sheet, which is critical for achieving high contrast, well resolved images, adds a significant challenge if fast functional imaging is also required, as multiple images need to be collected across several image planes. Consequently, fast functional imaging of neurons is typically restricted to a small tissue volume where part of the neuronal structure lies within the plane of a single image. Here we describe a method by which fast functional imaging can be achieved across a much larger tissue volume; a custom-built light sheet microscope is presented that includes a synchronized galvo mirror and electrically tunable lens, enabling high speed acquisition of images across a configurable depth. We assess the utility of this technique by acquiring fast functional Ca2+ imaging data across a neuron’s dendritic arbour in mammalian brain tissue.
Citation
Haslehurst , P , Yang , Z , Dholakia , K & Emptage , N 2018 , ' Fast volume-scanning light sheet microscopy reveals transient neuronal events ' , Biomedical Optics Express , vol. 9 , no. 5 , pp. 2154-2167 . https://doi.org/10.1364/BOE.9.002154
Publication
Biomedical Optics Express
Status
Peer reviewed
DOI
https://doi.org/10.1364/BOE.9.002154
ISSN
2156-7085
Type
Journal article
Rights
Copyright 2017 the Authors. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
Description
Funding: UK EPSRC EP/P030017/1.
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/13107

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