Light-sheet microscopy with attenuation-compensated propagation-invariant beams
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Scattering and absorption limit the penetration of optical fields into tissue. We demonstrate a new approach for increased depth penetration in light-sheet microscopy: attenuation-compensation of the light field. This tailors an exponential intensity increase along the illuminating propagation-invariant field, enabling the redistribution of intensity strategically within a sample to maximize signal and minimize irradiation. A key attribute of this method is that only minimal knowledge of the specimen transmission properties is required. We numerically quantify the imaging capabilities of attenuation-compensated Airy and Bessel light sheets, showing that increased depth penetration is gained without compromising any other beam attributes. This powerful yet straightforward concept, combined with the self-healing properties of the propagation-invariant field, improves the contrast-to-noise ratio of light-sheet microscopy up to eightfold across the entire field of view in thick biological specimens. This improvement can significantly increase the imaging capabilities of light-sheet microscopy techniques using Airy, Bessel, and other propagation-invariant beam types, paving the way for widespread uptake by the biomedical community.
Nylk , J , McCluskey , K A , Preciado , M , Mazilu , M , Yang , Z , Gunn-Moore , F J , Aggarwal , S , Tello , J A , Ferrier , D E K & Dholakia , K 2018 , ' Light-sheet microscopy with attenuation-compensated propagation-invariant beams ' , Science Advances , vol. 4 , no. 4 , eaar4817 . https://doi.org/10.1126/sciadv.aar4817
Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
DescriptionWe thank the UK Engineering and Physical Sciences Research Council for funding through grants (EP/P030017/1, EP/R004854/1, and EP/J01771X/1). J.A.T. acknowledges funding from the British Society for Neuroendocrinology Project Support Grant as well as the RS MacDonald Trust. D.E.K.F. acknowledges funding from the Leverhulme Trust.
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