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Light-sheet microscopy with attenuation-compensated propagation-invariant beams
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dc.contributor.author | Nylk, Jonathan | |
dc.contributor.author | McCluskey, Kaley Allyn | |
dc.contributor.author | Preciado, Miguel | |
dc.contributor.author | Mazilu, Michael | |
dc.contributor.author | Yang, Zhengyi | |
dc.contributor.author | Gunn-Moore, Francis James | |
dc.contributor.author | Aggarwal, Sanya | |
dc.contributor.author | Tello, Javier Ananda | |
dc.contributor.author | Ferrier, David Ellard Keith | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2018-04-10T11:30:07Z | |
dc.date.available | 2018-04-10T11:30:07Z | |
dc.date.issued | 2018-04-06 | |
dc.identifier | 252130861 | |
dc.identifier | cd917a7c-9b07-4fb0-af4c-b4b279b437c6 | |
dc.identifier | 85045022515 | |
dc.identifier | 000431374900056 | |
dc.identifier.citation | Nylk , J , McCluskey , K A , Preciado , M , Mazilu , M , Yang , Z , Gunn-Moore , F J , Aggarwal , S , Tello , J A , Ferrier , D E K & Dholakia , K 2018 , ' Light-sheet microscopy with attenuation-compensated propagation-invariant beams ' , Science Advances , vol. 4 , no. 4 , eaar4817 . https://doi.org/10.1126/sciadv.aar4817 | en |
dc.identifier.issn | 2375-2548 | |
dc.identifier.other | ORCID: /0000-0003-3247-6233/work/43646793 | |
dc.identifier.other | ORCID: /0000-0003-3422-3387/work/43646794 | |
dc.identifier.other | ORCID: /0000-0002-2977-4929/work/43646805 | |
dc.identifier.other | ORCID: /0000-0001-6637-2155/work/64034501 | |
dc.identifier.uri | https://hdl.handle.net/10023/13104 | |
dc.description | We thank the UK Engineering and Physical Sciences Research Council for funding through grants (EP/P030017/1, EP/R004854/1, and EP/J01771X/1). J.A.T. acknowledges funding from the British Society for Neuroendocrinology Project Support Grant as well as the RS MacDonald Trust. D.E.K.F. acknowledges funding from the Leverhulme Trust. | en |
dc.description.abstract | Scattering and absorption limit the penetration of optical fields into tissue. We demonstrate a new approach for increased depth penetration in light-sheet microscopy: attenuation-compensation of the light field. This tailors an exponential intensity increase along the illuminating propagation-invariant field, enabling the redistribution of intensity strategically within a sample to maximize signal and minimize irradiation. A key attribute of this method is that only minimal knowledge of the specimen transmission properties is required. We numerically quantify the imaging capabilities of attenuation-compensated Airy and Bessel light sheets, showing that increased depth penetration is gained without compromising any other beam attributes. This powerful yet straightforward concept, combined with the self-healing properties of the propagation-invariant field, improves the contrast-to-noise ratio of light-sheet microscopy up to eightfold across the entire field of view in thick biological specimens. This improvement can significantly increase the imaging capabilities of light-sheet microscopy techniques using Airy, Bessel, and other propagation-invariant beam types, paving the way for widespread uptake by the biomedical community. | |
dc.format.extent | 13 | |
dc.format.extent | 7285597 | |
dc.language.iso | eng | |
dc.relation.ispartof | Science Advances | en |
dc.subject | QC Physics | en |
dc.subject | QH301 Biology | en |
dc.subject | T Technology | en |
dc.subject | DAS | en |
dc.subject | BDC | en |
dc.subject | R2C | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | T | en |
dc.title | Light-sheet microscopy with attenuation-compensated propagation-invariant beams | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | British Society for Neuroendocrinology | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Cellular Medicine Division | en |
dc.contributor.institution | University of St Andrews. Marine Alliance for Science & Technology Scotland | en |
dc.contributor.institution | University of St Andrews. Scottish Oceans Institute | en |
dc.identifier.doi | 10.1126/sciadv.aar4817 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/P030017/1 | en |
dc.identifier.grantnumber | EP/P030017/1 | en |
dc.identifier.grantnumber | EP/R004854/1 | en |
dc.identifier.grantnumber | EP/J01771X/1 | en |
dc.identifier.grantnumber | EP/J01771X/1 | en |
dc.identifier.grantnumber | en |
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