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dc.contributor.authorNylk, Jonathan
dc.contributor.authorMcCluskey, Kaley Allyn
dc.contributor.authorPreciado, Miguel
dc.contributor.authorMazilu, Michael
dc.contributor.authorYang, Zhengyi
dc.contributor.authorGunn-Moore, Francis James
dc.contributor.authorAggarwal, Sanya
dc.contributor.authorTello, Javier Ananda
dc.contributor.authorFerrier, David Ellard Keith
dc.contributor.authorDholakia, Kishan
dc.identifier.citationNylk , J , McCluskey , K A , Preciado , M , Mazilu , M , Yang , Z , Gunn-Moore , F J , Aggarwal , S , Tello , J A , Ferrier , D E K & Dholakia , K 2018 , ' Light-sheet microscopy with attenuation-compensated propagation-invariant beams ' , Science Advances , vol. 4 , no. 4 , eaar4817 .
dc.identifier.otherPURE: 252130861
dc.identifier.otherPURE UUID: cd917a7c-9b07-4fb0-af4c-b4b279b437c6
dc.identifier.otherScopus: 85045022515
dc.identifier.otherORCID: /0000-0003-3247-6233/work/43646793
dc.identifier.otherORCID: /0000-0003-3422-3387/work/43646794
dc.identifier.otherORCID: /0000-0002-2977-4929/work/43646805
dc.identifier.otherWOS: 000431374900056
dc.identifier.otherORCID: /0000-0001-6637-2155/work/64034501
dc.descriptionWe thank the UK Engineering and Physical Sciences Research Council for funding through grants (EP/P030017/1, EP/R004854/1, and EP/J01771X/1). J.A.T. acknowledges funding from the British Society for Neuroendocrinology Project Support Grant as well as the RS MacDonald Trust. D.E.K.F. acknowledges funding from the Leverhulme Trust.en
dc.description.abstractScattering and absorption limit the penetration of optical fields into tissue. We demonstrate a new approach for increased depth penetration in light-sheet microscopy: attenuation-compensation of the light field. This tailors an exponential intensity increase along the illuminating propagation-invariant field, enabling the redistribution of intensity strategically within a sample to maximize signal and minimize irradiation. A key attribute of this method is that only minimal knowledge of the specimen transmission properties is required. We numerically quantify the imaging capabilities of attenuation-compensated Airy and Bessel light sheets, showing that increased depth penetration is gained without compromising any other beam attributes. This powerful yet straightforward concept, combined with the self-healing properties of the propagation-invariant field, improves the contrast-to-noise ratio of light-sheet microscopy up to eightfold across the entire field of view in thick biological specimens. This improvement can significantly increase the imaging capabilities of light-sheet microscopy techniques using Airy, Bessel, and other propagation-invariant beam types, paving the way for widespread uptake by the biomedical community.
dc.relation.ispartofScience Advancesen
dc.rightsCopyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).en
dc.subjectQC Physicsen
dc.subjectQH301 Biologyen
dc.subjectT Technologyen
dc.titleLight-sheet microscopy with attenuation-compensated propagation-invariant beamsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Cellular Medicine Divisionen
dc.contributor.institutionUniversity of St Andrews.Marine Alliance for Science & Technology Scotlanden
dc.contributor.institutionUniversity of St Andrews.Scottish Oceans Instituteen
dc.description.statusPeer revieweden

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