The major histocompatibility complex class I immunopeptidome of extracellular vesicles
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Extracellular vesicles (EVs) are released by most cell types and have been associated with multiple immunomodulatory functions. MHC class I molecules have crucial roles in antigen presentation and in eliciting immune responses and are known to be incorporated into EVs. However, the MHC class Iimmunopeptidome of EVs has not been established. Here, using a small-scale immunoisolation of the antigen serotypes HLA-A*02:01 and HLA-B*27:05 expressed on the Epstein–Barr virus–transformed B cell line Jesthom and MS of the eluted peptides from both cells and EVs, we identified 516 peptides that bind either HLA-A*02:01 orHLA-B*27:05. Of importance, the predicted serotype-binding affinities and peptide-anchor motifs did not significantly differ between the peptide pools isolated from cells or EVs,indicating that during EV biogenesis, no obvious editing of the MHC class Iimmunopeptidome occurs. These results, for the first time, establish EVs as a source ofMHC class I peptides that can be used for the study of the immunopeptidome and in the discovery of potential neoantigens for immunotherapies.
Synowsky , S A , Shirran , S L , Cooke , F G M , Antoniou , A N , Botting , C H & Powis , S J 2017 , ' The major histocompatibility complex class I immunopeptidome of extracellular vesicles ' Journal of Biological Chemistry , vol Papers in Press . DOI: 10.1074/jbc.M117.805895
Journal of Biological Chemistry
© 2017, American Society for Biochemistry and Molecular Biology, Inc. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at www.jbc.org / https://doi.org/10.1074/jbc.M117.805895
This work was funded by the Melville Trust for the Care and Cure of Cancer, Scotland, UK.
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