Integrated single- and two-photon light sheet microscopy using accelerating beams
MetadataShow full item record
We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.
Piksarv , P , Marti , D , Le , T , Unterhuber , A , Forbes , L H , Andrews , M R , Stingl , A , Drexler , W , Andersen , P E & Dholakia , K 2017 , ' Integrated single- and two-photon light sheet microscopy using accelerating beams ' , Scientific Reports , vol. 7 , 1435 . https://doi.org/10.1038/s41598-017-01543-4
Copyright 2017 the Authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
DescriptionThe authors thank the UK Engineering and Physical Sciences Research Council (grant EP/J01771/X) and the European Union project FAMOS (FP7 ICT, contract no. 317744) for funding. P.P. acknowledges funding by the Estonian Research Council (grants PUTJD8 and PUT369).
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.