Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorTakei, Yoshio
dc.contributor.authorSuzuki, Ippei
dc.contributor.authorWong, Marty K. S.
dc.contributor.authorMilne, Ryan
dc.contributor.authorMoss, Simon
dc.contributor.authorSato, Katsufumi
dc.contributor.authorHall, Ailsa
dc.date.accessioned2017-08-15T08:44:41Z
dc.date.available2017-08-15T08:44:41Z
dc.date.issued2016-10-06
dc.identifier.citationTakei , Y , Suzuki , I , Wong , M K S , Milne , R , Moss , S , Sato , K & Hall , A 2016 , ' Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals ' , American Journal of Physiology. Regulatory, Integrative and Comparative Physiology , vol. 311 , no. 4 , pp. R788-R796 . https://doi.org/10.1152/ajpregu.00211.2016en
dc.identifier.issn0363-6119
dc.identifier.otherPURE: 247145839
dc.identifier.otherPURE UUID: 0339f705-f833-47da-ab69-c2f4bd22e525
dc.identifier.otherScopus: 84991013865
dc.identifier.otherORCID: /0000-0002-7562-1771/work/47136289
dc.identifier.otherWOS: 000388456500020
dc.identifier.urihttp://hdl.handle.net/10023/11456
dc.descriptionThe research was supported by Bilateral Program between Japan and the United Kingdom and a Grant-in-Aid for Scientific Research (23247010) to Y. Takei and by Grant-in-Aid for challenging Exploratory Research (15K14567) to I. Suzuki from the Japan Society for the Promotion of Science. This work was also supported by funding from the U.K. Natural Environment Research Council (Grant SMRU1001).en
dc.description.abstractAn animal-borne blood sampler with data-logging functions was developed for phocid seals, which collected two blood samples for the comparison of endocrino-logical/biochemical parameters under two different conditions. The sampler can be triggered by preset hydrostatic pressure, acceleration (descending or ascending), temperature, and time, and also man-ually by light. The sampling was reliable with 39/50 (78%) successful attempts to collect blood samples. Contamination of fluids in the tubing to the next blood sample was 1%, following the prior clearance of the tubing to a waste syringe. In captive harbor seals (Phoca vitulina), the automated blood-sampling method was less stressful than direct blood withdrawal, as evidenced by lower levels of stress hormones (P < 0.05 for ACTH and P = 0.078 for cortisol). HPLC analyses showed that both cortisol and cortisone were circu-lating in seal blood. Using the sampler, plasma levels of cardiovascular hormones, atrial natriuretic peptide (ANP), AVP, and ANG II were compared in grey seals (Halichoerus grypus), between samples collected when the animals were on land and in the water. HPLC analyses determined that [Met12] ANP (1-28) and various forms of angiotensins (ANG II, III, and IV) were circulating in seal blood. Although water immersion profoundly changes the plasma levels of cardiovascular hormones in terrestrial mammals, there were only tendencies toward an increase in ANP (P = 0.069) and a decrease in AVP (P = 0.074) in the seals. These results suggest that cardiovascular regulation in phocid seals may have undergone adaptation during evolution of the carnivore to a semiaquatic lifestyle.
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofAmerican Journal of Physiology. Regulatory, Integrative and Comparative Physiologyen
dc.rights© 2016, American Physiological Society. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at ajpregu.physiology.org / https://doi.org/10.1152/ajpregu.00211.2016en
dc.subjectCardiovascular hormoneen
dc.subjectData loggeren
dc.subjectGravityen
dc.subjectSea mammalen
dc.subjectStress hormoneen
dc.subjectQH301 Biologyen
dc.subjectQP Physiologyen
dc.subjectPhysiologyen
dc.subjectPhysiology (medical)en
dc.subjectNDASen
dc.subjectBDCen
dc.subject.lccQH301en
dc.subject.lccQPen
dc.titleDevelopment of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid sealsen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.Sea Mammal Research Uniten
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Marine Alliance for Science & Technology Scotlanden
dc.contributor.institutionUniversity of St Andrews.Scottish Oceans Instituteen
dc.identifier.doihttps://doi.org/10.1152/ajpregu.00211.2016
dc.description.statusPeer revieweden
dc.date.embargoedUntil2017-08-01


This item appears in the following Collection(s)

Show simple item record