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Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals

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AJP_R_00211_2016_Accepted.pdf (789.1Kb)
Date
06/10/2016
Author
Takei, Yoshio
Suzuki, Ippei
Wong, Marty K. S.
Milne, Ryan
Moss, Simon
Sato, Katsufumi
Hall, Ailsa
Funder
NERC
Grant ID
Agreement R8-H12-86
Keywords
Cardiovascular hormone
Data logger
Gravity
Sea mammal
Stress hormone
QH301 Biology
QP Physiology
Physiology
Physiology (medical)
NDAS
BDC
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Abstract
An animal-borne blood sampler with data-logging functions was developed for phocid seals, which collected two blood samples for the comparison of endocrino-logical/biochemical parameters under two different conditions. The sampler can be triggered by preset hydrostatic pressure, acceleration (descending or ascending), temperature, and time, and also man-ually by light. The sampling was reliable with 39/50 (78%) successful attempts to collect blood samples. Contamination of fluids in the tubing to the next blood sample was 1%, following the prior clearance of the tubing to a waste syringe. In captive harbor seals (Phoca vitulina), the automated blood-sampling method was less stressful than direct blood withdrawal, as evidenced by lower levels of stress hormones (P < 0.05 for ACTH and P = 0.078 for cortisol). HPLC analyses showed that both cortisol and cortisone were circu-lating in seal blood. Using the sampler, plasma levels of cardiovascular hormones, atrial natriuretic peptide (ANP), AVP, and ANG II were compared in grey seals (Halichoerus grypus), between samples collected when the animals were on land and in the water. HPLC analyses determined that [Met12] ANP (1-28) and various forms of angiotensins (ANG II, III, and IV) were circulating in seal blood. Although water immersion profoundly changes the plasma levels of cardiovascular hormones in terrestrial mammals, there were only tendencies toward an increase in ANP (P = 0.069) and a decrease in AVP (P = 0.074) in the seals. These results suggest that cardiovascular regulation in phocid seals may have undergone adaptation during evolution of the carnivore to a semiaquatic lifestyle.
Citation
Takei , Y , Suzuki , I , Wong , M K S , Milne , R , Moss , S , Sato , K & Hall , A 2016 , ' Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals ' , American Journal of Physiology. Regulatory, Integrative and Comparative Physiology , vol. 311 , no. 4 , pp. R788-R796 . https://doi.org/10.1152/ajpregu.00211.2016
Publication
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Status
Peer reviewed
DOI
https://doi.org/10.1152/ajpregu.00211.2016
ISSN
0363-6119
Type
Journal article
Rights
© 2016, American Physiological Society. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at ajpregu.physiology.org / https://doi.org/10.1152/ajpregu.00211.2016
Description
The research was supported by Bilateral Program between Japan and the United Kingdom and a Grant-in-Aid for Scientific Research (23247010) to Y. Takei and by Grant-in-Aid for challenging Exploratory Research (15K14567) to I. Suzuki from the Japan Society for the Promotion of Science. This work was also supported by funding from the U.K. Natural Environment Research Council (Grant SMRU1001).
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/11456

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