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DNA‐Interacting characteristics of the archaeal Rudiviral protein SIRV2_Gp1

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viruses_09_00190.pdf (3.654Mb)
Date
07/2017
Author
Peeters, Eveline
Boon, Maarten
Rollie, Clare Jane Catherine
Willaert, Ronnie G.
Voet, Marleen
White, Malcolm Frederick
Prangishvili, David
Lavigne, Rob
Quax, Tessa E. F.
Funder
BBSRC
Grant ID
BB/M021017/1
Keywords
Archaea
Archaeal virus
Rudiviridae
SIRV2
Solfolobus
DNA binding
Helix-turn-helix doman
QH301 Biology
QR355 Virology
NDAS
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Abstract
Whereas the infection cycles of many bacterial and eukaryotic viruses have been characterized in detail, those of archaeal viruses remain largely unexplored. Recently, studies on a few model archaeal viruses such as SIRV2 (Sulfolobus islandicus rod‐shaped virus) have revealed an unusual lysis mechanism that involves the formation of pyramidal egress structures on the host cell surface. To expand understanding of the infection cycle of SIRV2, we aimed to functionally characterize gp1, which is a SIRV2 gene with unknown function. The SIRV2_Gp1 protein is highly expressed during early stages of infection and it is the only protein that is encoded twice on the viral genome. It harbours a helix‐turn‐helix motif and was therefore hypothesized to bind DNA. The DNA‐binding behavior of SIRV2_Gp1 was characterized with electrophoretic mobility shift assays and atomic force microscopy. We provide evidence that the protein interacts with DNA and that it forms large aggregates, thereby causing extreme condensation of the DNA. Furthermore, the N‐terminal domain of the protein mediates toxicity to the viral host Sulfolobus. Our findings may lead to biotechnological applications, such as the development of a toxic peptide for the containment of pathogenic bacteria, and add to our understanding of the Rudiviral infection cycle.
Citation
Peeters , E , Boon , M , Rollie , C J C , Willaert , R G , Voet , M , White , M F , Prangishvili , D , Lavigne , R & Quax , T E F 2017 , ' DNA‐Interacting characteristics of the archaeal Rudiviral protein SIRV2_Gp1 ' , Viruses , vol. 9 , no. 7 , 190 . https://doi.org/10.3390/v9070190
Publication
Viruses
Status
Peer reviewed
DOI
https://doi.org/10.3390/v9070190
ISSN
1999-4915
Type
Journal article
Rights
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/11250

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

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