Identifying bird remains using ancient DNA barcoding
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Bird remains that are difficult to identify taxonomically using morphological methods, are common in the palaeontological record. Other types of challenging avian material include artefacts and food items from endangered taxa, as well as remains from aircraft strikes. We here present a DNA-based method that enables taxonomic identification of bird remains, even from material where the DNA is heavily degraded. The method is based on the amplification and sequencing of two short variable parts of the 16S region in the mitochondrial genome. To demonstrate the applicability of this approach, we evaluated the method on a set of Holocene and Late Pleistocene postcranial bird bones from several palaeontological and archaeological sites in Europe with good success.
Dalén , L , Lagerholm , V K , Nylander , J A A , Barton , N , Bochenski , Z M , Tomek , T , Rudling , D , Ericson , P G P , Irestedt , M & Stewart , J R 2017 , ' Identifying bird remains using ancient DNA barcoding ' , Genes , vol. 8 , no. 6 , 169 . https://doi.org/10.3390/genes8060169
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
DescriptionThis project was made possible through a grant (SE-TAF-3037) to J.R.S. from the EU-funded SYNTHESYS project. L.D. also acknowledges support from the Swedish Research Council.
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