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Identifying bird remains using ancient DNA barcoding

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Lagerholm_2017_Genes_BirdRemains_CC.pdf (229.6Kb)
Date
21/06/2017
Author
Dalén, Love
Lagerholm, Vendela K.
Nylander, Johan A. A.
Barton, Nick
Bochenski, Zbigniew M.
Tomek, Teresa
Rudling, David
Ericson, Per G. P.
Irestedt, Martin
Stewart, John R.
Keywords
16S
Archaeology
Aves
Biodiversity
Palaeogenetics
Palaeontology
Species identification
QH301 Biology
QH426 Genetics
Genetics
Genetics(clinical)
NDAS
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Abstract
Bird remains that are difficult to identify taxonomically using morphological methods, are common in the palaeontological record. Other types of challenging avian material include artefacts and food items from endangered taxa, as well as remains from aircraft strikes. We here present a DNA-based method that enables taxonomic identification of bird remains, even from material where the DNA is heavily degraded. The method is based on the amplification and sequencing of two short variable parts of the 16S region in the mitochondrial genome. To demonstrate the applicability of this approach, we evaluated the method on a set of Holocene and Late Pleistocene postcranial bird bones from several palaeontological and archaeological sites in Europe with good success.
Citation
Dalén , L , Lagerholm , V K , Nylander , J A A , Barton , N , Bochenski , Z M , Tomek , T , Rudling , D , Ericson , P G P , Irestedt , M & Stewart , J R 2017 , ' Identifying bird remains using ancient DNA barcoding ' , Genes , vol. 8 , no. 6 , 169 . https://doi.org/10.3390/genes8060169
Publication
Genes
Status
Peer reviewed
DOI
https://doi.org/10.3390/genes8060169
ISSN
2073-4425
Type
Journal article
Rights
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Description
This project was made possible through a grant (SE-TAF-3037) to J.R.S. from the EU-funded SYNTHESYS project. L.D. also acknowledges support from the Swedish Research Council.
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  • University of St Andrews Research
URL
http://www.mdpi.com/2073-4425/8/6/169#supplementary
URI
http://hdl.handle.net/10023/11168

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