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dc.contributor.authorBalao, Francisco
dc.contributor.authorTrucchi, Emiliano
dc.contributor.authorWolfe, Thomas
dc.contributor.authorHao, Bao-Hai
dc.contributor.authorLorenzo, Maria Teresa
dc.contributor.authorBaar, Juliane
dc.contributor.authorSedman, Laura
dc.contributor.authorKosiol, Carolin
dc.contributor.authorAmman, Fabian
dc.contributor.authorChase, Mark W.
dc.contributor.authorHedrén, Mikael
dc.contributor.authorPaun, Ovidiu
dc.identifier.citationBalao , F , Trucchi , E , Wolfe , T , Hao , B-H , Lorenzo , M T , Baar , J , Sedman , L , Kosiol , C , Amman , F , Chase , M W , Hedrén , M & Paun , O 2017 , ' Adaptive sequence evolution is driven by biotic stress in a pair of orchid species ( Dactylorhiza ) with distinct ecological optima ' , Molecular Ecology , vol. 26 , no. 14 , pp. 3649-3662 .
dc.identifier.otherPURE: 249511268
dc.identifier.otherPURE UUID: 7310a57a-142f-4511-a0de-fc434810f053
dc.identifier.otherBibtex: urn:bed6e01db81a9d428a87d0d3ec9bfec1
dc.identifier.otherScopus: 85018991642
dc.identifier.otherWOS: 000404618000007
dc.descriptionThis work was funded by an Austrian Science Fund (FWF) project (Y661-B16) awarded to OP and a Marie Curie IEF fellowship (PERG-GA-2011-299608-TRANSADAPTATION) to FB.en
dc.description.abstractThe orchid family is the largest in the angiosperms, but little is known about the molecular basis of the significant variation they exhibit. We investigate here the transcriptomic divergence between two European terrestrial orchids, Dactylorhiza incarnata and D. fuchsii, and integrate these results in the context of their distinct ecologies that we also document. Clear signals of lineage-specific adaptive evolution of protein-coding sequences are identified, notably targeting elements of biotic defence, including both physical and chemical adaptations in the context of divergent pools of pathogens and herbivores. In turn, a substantial regulatory divergence between the two species appears linked to adaptation/acclimation to abiotic conditions. Several of the pathways affected by differential expression are also targeted by deviating post-transcriptional regulation via sRNAs. Finally, Dactylorhiza incarnata appears to suffer from insufficient sRNA control over the activity of RNA-dependent DNA polymerase, resulting in increased activity of class I transposable elements and, over time, in larger genome size than that of D. fuchsii. The extensive molecular divergence between the two species suggests significant genomic and transcriptomic shock in their hybrids and offers insights into the difficulty of coexistence at the homoploid level. Altogether, biological response to selection, accumulated during the history of these orchids, appears governed by their microenvironmental context, in which biotic and abiotic pressures act synergistically to shape transcriptome structure, expression and regulation.
dc.relation.ispartofMolecular Ecologyen
dc.rights© 2017 The Authors. Molecular Ecology Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium,provided the original work is properly cited.en
dc.subjectAbiotic stressen
dc.subjectEcological divergenceen
dc.subjectPositive selectionen
dc.subjectSmall RNAsen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectQK Botanyen
dc.titleAdaptive sequence evolution is driven by biotic stress in a pair of orchid species (Dactylorhiza) with distinct ecological optimaen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Centre for Biological Diversityen
dc.description.statusPeer revieweden

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