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dc.contributor.authorPullman, James
dc.contributor.authorNylk, Jonathan
dc.contributor.authorCampbell, Elaine Catherine
dc.contributor.authorGunn-Moore, Frank J
dc.contributor.authorPrystowsky, Michael B
dc.contributor.authorDholakia, Kishan
dc.date.accessioned2017-01-07T00:32:12Z
dc.date.available2017-01-07T00:32:12Z
dc.date.issued2016-02-01
dc.identifier.citationPullman , J , Nylk , J , Campbell , E C , Gunn-Moore , F J , Prystowsky , M B & Dholakia , K 2016 , ' Visualization of podocyte substructure with structured illumination microscopy (SIM) : a new approach to nephrotic disease ' , Biomedical Optics Express , vol. 7 , no. 2 , pp. 302-311 . https://doi.org/10.1364/BOE.7.000302en
dc.identifier.issn2156-7085
dc.identifier.otherPURE: 240105263
dc.identifier.otherPURE UUID: 5a8ef23c-5adb-4a7e-9d52-0c99eec38147
dc.identifier.otherScopus: 84961859572
dc.identifier.otherORCID: /0000-0003-3422-3387/work/34730416
dc.identifier.otherORCID: /0000-0002-2977-4929/work/33493303
dc.identifier.otherWOS: 000369247000006
dc.identifier.urihttp://hdl.handle.net/10023/10065
dc.descriptionWe thank the UK Engineering and Physical Sciences Research Council under grant EP/J0177X/1, the RS Macdonald Charitable Trust, the BRAINS 600th anniversary appeal, and Dr. Killick for funding. KD acknowledges the award of a Royal Society Leverhulme Trust Senior Fellowship.en
dc.description.abstractA detailed microscopic analysis of renal podocyte substructure is essential to understand and diagnose nephrotic kidney disease. Currently only time consuming electron microscopy (EM) can resolve this substructure. We used structured illumination microscopy (SIM) to examine frozen sections of renal biopsies stained with an immunofluorescence marker for podocin, a protein localized to the perimeter of the podocyte foot processes and compared them with EM in both normal and nephrotic disease biopsies. SIM images of normal glomeruli revealed curvilinear patterns of podocin densely covering capillary walls similar to podocyte foot processes seen by EM. Podocin staining of all nephrotic disease biopsies were significantly different than normal, corresponding to and better visualizing effaced foot processes seen by EM. The findings support the first potential use of SIM in the diagnosis of nephrotic disease.
dc.language.isoeng
dc.relation.ispartofBiomedical Optics Expressen
dc.rightsCopyright 2016 OSA. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at: https://dx.doi.org/10.1364/BOE.7.000302. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.en
dc.subjectQH301 Biologyen
dc.subjectQC Physicsen
dc.subjectDASen
dc.subject.lccQH301en
dc.subject.lccQCen
dc.titleVisualization of podocyte substructure with structured illumination microscopy (SIM) : a new approach to nephrotic diseaseen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1364/BOE.7.000302
dc.description.statusPeer revieweden
dc.date.embargoedUntil2017-01-06


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