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dc.contributor.authorVigoder, Felipe M.
dc.contributor.authorParker, Darren James
dc.contributor.authorCook, Nicola
dc.contributor.authorTournière, Océane
dc.contributor.authorSneddon, Tanya
dc.contributor.authorRitchie, Michael Gordon
dc.date.accessioned2016-11-11T09:30:14Z
dc.date.available2016-11-11T09:30:14Z
dc.date.issued2016-11-10
dc.identifier.citationVigoder , F M , Parker , D J , Cook , N , Tournière , O , Sneddon , T & Ritchie , M G 2016 , ' Inducing cold-sensitivity in the frigophilic fly Drosophila montana by RNAi ' PLoS One , vol. 11 , no. 11 , e0165724 . https://doi.org/10.1371/journal.pone.0165724en
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 247455510
dc.identifier.otherPURE UUID: 882caca0-0202-43e0-8be6-4dc501e59e12
dc.identifier.otherScopus: 84994796533
dc.identifier.otherORCID: /0000-0001-7913-8675/work/46761139
dc.identifier.otherORCID: /0000-0003-4462-0116/work/60427612
dc.identifier.otherWOS: 000387725000030
dc.identifier.urihttp://hdl.handle.net/10023/9804
dc.descriptionThe work was supported by CNPq (Fellowship to FMV) and a NERC Studentship to DJP.en
dc.description.abstractCold acclimation is a critical physiological adaptation for coping with seasonal cold. By increasing their cold tolerance individuals can remain active for longer at the onset of winter and can recover more quickly from a cold shock. In insects, despite many physiological studies, little is known about the genetic basis of cold acclimation. Recently, transcriptomic analyses in Drosophila virilis and D.montana revealed candidate genes for cold acclimation by identifying genes upregulated during exposure to cold. Here, we test the role of myo-inositol-1-phosphate synthase (Inos), in cold tolerance in D. montana using an RNAi approach. D. montana has a circumpolar distribution and overwinters as an adult in northern latitudes with extreme cold. We assessed cold tolerance of dsRNA knock-down flies using two metrics: chill-coma recovery time (CCRT) and mortality rate after cold acclimation. Injection of dsRNAInos did not alter CCRT,either overall or in interaction with the cold treatment, however it did induced cold specific mortality, with high levels of mortality observed in injected flies acclimated at 5°C but not at 19°C. Overall, injection with dsRNAInos induced a temperature sensitive mortality rate of over 60% in this normally cold-tolerant species. qPCRanalysis confirmed that dsRNA injection successfully reduced gene expression of Inos. Thus, our results demonstrate the involvement of Inos in increasing cold tolerance in D. montana. The potential mechanisms involved by which Inos increases cold tolerance are also discussed.
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofPLoS Oneen
dc.rights© 2016 Vigoder et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectDrosophiliaen
dc.subjectCold toleranceen
dc.subjectRNAien
dc.subjectThermal adaptationen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.titleInducing cold-sensitivity in the frigophilic fly Drosophila montana by RNAien
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.Centre for Biological Diversityen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0165724
dc.description.statusPeer revieweden


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