Show simple item record

Files in this item


Item metadata

dc.contributor.authorSpesyvtsev, Roman
dc.contributor.authorRendall, Helen Anne
dc.contributor.authorDholakia, Kishan
dc.identifier.citationSpesyvtsev , R , Rendall , H A & Dholakia , K 2015 , ' Wide-field 3D optical imaging using temporal focusing for holographically trapped microparticles ' , Optics Letters , vol. 40 , no. 21 , pp. 4847-4850 .
dc.identifier.otherPURE: 224808893
dc.identifier.otherPURE UUID: 80861c4c-11bf-4c7f-a9b4-06a57e1d5778
dc.identifier.otherScopus: 84957543476
dc.identifier.otherWOS: 000364468600007
dc.descriptionFunding. Engineering and Physical Sciences Research Council (EPSRC) (EP/J01771X/1, EP/M000869/1).en
dc.description.abstractA contemporary challenge across the natural sciences is the simultaneous optical imaging or stimulation of small numbers of cells or colloidal particles organised into arbitrary geometries. We demonstrate the use of temporal focusing with holographic optical tweezers in order to achieve depth-resolved two-photon imaging of trapped objects arranged in arbitrary three dimensional geometries using a single objective. Trapping allows independent position control of multiple objects by holographic beam shaping. Temporal focusing of ultrashort pulses providesawide-field two-photon depth-selective activation of fluorescent samples. We demonstrate wide-field depth-resolved illumination of both trapped fluorescent beads and trapped HL60 cells in suspension with full 3D positioning control. These approaches are compatible with implementation through scattering media and can be beneficial for emergent studies in colloidal science and particularly optogenetics, offering targeted photoactivation over a wide area with µm depth control precision.
dc.relation.ispartofOptics Lettersen
dc.rights© 2015 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.en
dc.subjectQB Astronomyen
dc.subjectQC Physicsen
dc.titleWide-field 3D optical imaging using temporal focusing for holographically trapped microparticlesen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

This item appears in the following Collection(s)

Show simple item record