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dc.contributor.authorLadomery, Michael
dc.contributor.authorSommerville, John
dc.date.accessioned2016-10-09T23:33:35Z
dc.date.available2016-10-09T23:33:35Z
dc.date.issued2015
dc.identifier.citationLadomery , M & Sommerville , J 2015 , ' The Scd6/Lsm14 protein xRAPB has properties different from RAP55 in selecting mRNA for early translation or intracellular distribution in Xenopus oocytes ' Biochimica et Biophysica Acta - Gene regulatory mechanisms , vol. In press . DOI: 10.1016/j.bbagrm.2015.10.002en
dc.identifier.issn1874-9399
dc.identifier.otherPURE: 222571015
dc.identifier.otherPURE UUID: be7a968b-85b5-448e-bc1f-713797bebaec
dc.identifier.otherRIS: urn:CCEFB424B27689E284750A0E57531E42
dc.identifier.otherScopus: 84945205467
dc.identifier.urihttp://hdl.handle.net/10023/9632
dc.descriptionThe project was supported by The Wellcome Trust.en
dc.description.abstractAbstract Oocytes accumulate mRNAs in the form of maternal ribonucleoprotein (RNP) particles, the protein components of which determine the location and stability of individual mRNAs prior to translation. Scd6/Lsm14 proteins, typified by RAP55, function in a wide range of eukaryotes in repressing translation and relocating mRNPs to processing bodies and stress granules. In Xenopus laevis, the RAP55 orthologue xRAPA fulfills these functions. Here we describe the properties of a variant of xRAPA, xRAPB, which is a member of the Lsm14B group. xRAPB differs from xRAPA in various respects: it is expressed at high concentration earlier in oogenesis; it interacts specifically with the DDX6 helicase Xp54; it is detected in polysomes and stalled translation initiation complexes; its over-expression leads to selective binding to translatable mRNA species without evidence of translation repression or mRNA degradation. Since both Xp54 and xRAPA are repressors of translation, activation appears to be effected through targeting of xRAPB/Xp54.en
dc.language.isoeng
dc.relation.ispartofBiochimica et Biophysica Acta - Gene regulatory mechanismsen
dc.rightsCopyright © 2015 Elsevier B.V. All rights reserved. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at: https://dx.doi.org/10.1016/j.bbagrm.2015.10.002en
dc.subjectMessenger ribonucleoprotein particles (mRNPs)en
dc.subjectScd6/RAP55 proteinsen
dc.subjectLsm14b-aen
dc.subjectXp54/DDX6 helicaseen
dc.subjectPost-transcripional controlen
dc.subjectXenopus oogenesisen
dc.subjectQH301 Biologyen
dc.subject.lccQH301en
dc.titleThe Scd6/Lsm14 protein xRAPB has properties different from RAP55 in selecting mRNA for early translation or intracellular distribution in Xenopus oocytesen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doihttps://doi.org/10.1016/j.bbagrm.2015.10.002
dc.description.statusPeer revieweden
dc.date.embargoedUntil09-10-20


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