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dc.contributor.authorGiroux, Xavier
dc.contributor.authorMacNeill, Stuart
dc.date.accessioned2016-09-29T23:34:43Z
dc.date.available2016-09-29T23:34:43Z
dc.date.issued2015
dc.identifier.citationGiroux , X & MacNeill , S 2015 , ' Inhibiting NAD + -dependent DNA ligase activity with 2-(cyclopentyloxy)-5'-deoxyadenosine (CPOdA) offers a new tool for DNA replication and repair studies in the model archaeon Haloferax volcanii ' , FEMS Microbiology Letters , vol. 362 , no. 21 , fnv181 . https://doi.org/10.1093/femsle/fnv181en
dc.identifier.issn0378-1097
dc.identifier.otherPURE: 219971059
dc.identifier.otherPURE UUID: eff2efa1-92be-4c30-92dc-693ff65b8009
dc.identifier.otherScopus: 84959549254
dc.identifier.otherORCID: /0000-0002-0555-0007/work/39107875
dc.identifier.otherWOS: 000366366700010
dc.identifier.urihttp://hdl.handle.net/10023/9580
dc.description.abstractDNA ligases play an essential role in many aspects of DNA metabolism in all three domains of life. The haloarchaeal organism Haloferax volcanii encodes both ATP- and NAD+-dependent DNA ligase enzymes designated LigA and LigN, respectively. Neither LigA nor LigN alone is required for cell viability but they share an essential function, most likely the ligation of Okazaki fragments during chromosome replication. Here we show that 2-(cyclopentyloxy)-5’-deoxyadenosine (referred to as CPOdA), originally developed as a inhibitor of bacterial NAD+-dependent DNA ligases, is a potent inhibitor of the growth of Hfx. volcanii cells expressing LigN alone, causing chromosome fragmentation and cell death, while cells expressing LigA are unaffected. Growth inhibition occurs at significantly lower CPOdA concentrations (MIC ≤ 50 ng/ml) than those required for inhibition of bacterial growth (≥ 2 µg/ml). CPOdA has the potential to become a vital tool in DNA replication and repair studies in this important model organism.
dc.format.extent5
dc.language.isoeng
dc.relation.ispartofFEMS Microbiology Lettersen
dc.rights© 2015, Publisher / the Author(s). This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at femsle.oxfordjournals.org / https://dx.doi.org/10.1093/femsle/fnv181en
dc.subjectDNA ligaseen
dc.subjectHaloferax volcaniien
dc.subjectArchaeaen
dc.subjectDNA replicationen
dc.subjectDNA repairen
dc.subjectQR Microbiologyen
dc.subject.lccQRen
dc.titleInhibiting NAD+-dependent DNA ligase activity with 2-(cyclopentyloxy)-5'-deoxyadenosine (CPOdA) offers a new tool for DNA replication and repair studies in the model archaeon Haloferax volcaniien
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1093/femsle/fnv181
dc.description.statusPeer revieweden
dc.date.embargoedUntil2016-09-29


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