Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue
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We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.
Nylk , J , McCluskey , K A , Aggarwal , S , Tello , J & Dholakia , K 2016 , ' Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue ' , Biomedical Optics Express , vol. 7 , no. 10 , pp. 4021-4033 . https://doi.org/10.1364/BOE.7.004021
Biomedical Optics Express
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